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Suck, K; Roeker, S; Diederichs, S; Anton, F; Sanz-Herrera, JA; Ochoa, I; Doblare, M; Scheper, T; van Griensven, M; Kasper, C.
(2010): A rotating bed system bioreactor enables cultivation of primary osteoblasts on well-characterized Sponceram regarding structural and flow properties.
Biotechnol Prog. 2010; 26(3):671-678 FullText FullText_BOKU

The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram (R), a ZrO(2) based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 671-678, 2010
Authors BOKU Wien:
Almeria Ciarra
Kasper Cornelia
Find related publications in this database (using NML MeSH Indexing)
Adult -
Biomechanics -
Bioreactors -
Ceramics -
Collagen - metabolism
Elastic Modulus -
Humans -
Male -
Materials Testing - instrumentation
Microscopy, Electron, Scanning -
Osteoblasts - cytology
Osteopontin - metabolism
Porosity -
Reverse Transcriptase Polymerase Chain Reaction -
Tissue Culture Techniques - methods
Tissue Engineering - instrumentation
Tissue Scaffolds - chemistry
X-Ray Microtomography -
Zirconium -

Find related publications in this database (Keywords)
Sponceram (R)
rotating bed system
primary osteoblasts
Youngxxxs modulus

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