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Schmeitzl, C; Warth, B; Fruhmann, P; Michlmayr, H; Malachov√°, A; Berthiller, F; Schuhmacher, R; Krska, R; Adam, G; .
(2015): The Metabolic Fate of Deoxynivalenol and Its Acetylated Derivatives in a Wheat Suspension Culture: Identification and Detection of DON-15-O-Glucoside, 15-Acetyl-DON-3-O-Glucoside and 15-Acetyl-DON-3-Sulfate.
Toxins (Basel). 2015; 7(8):311-326 FullText FullText_BOKU

Abstract:
Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption. We treated a wheat suspension culture with DON or one of its acetylated derivatives, 3-acetyl-DON (3-ADON), 15-acetyl-DON (15-ADON) and 3,15-diacetyl-DON (3,15-diADON), and monitored the metabolization over a course of 96 h. Supernatant and cell extract samples were analyzed using a tailored LC-MS/MS method for the quantification of DON metabolites. We report the formation of tentatively identified DON-15-O-beta-D-glucoside (D15G) and of 15-acetyl-DON-3-sulfate (15-ADON3S) as novel deoxynivalenol metabolites in wheat. Furthermore, we found that the recently identified 15-acetyl-DON-3-O-beta-D-glucoside (15-ADON3G) is the major metabolite produced after 15-ADON challenge. 3-ADON treatment led to a higher intracellular content of toxic metabolites after six hours compared to all other treatments. 3-ADON was exclusively metabolized into DON before phase II reactions occurred. In contrast, we found that 15-ADON was directly converted into 15-ADON3G and 15-ADON3S in addition to metabolization into deoxynivalenol-3-O-beta-D-glucoside (D3G). This study highlights significant differences in the metabolization of DON and its acetylated derivatives.
Authors BOKU Wien:
Adam Gerhard
Berthiller Franz
Krska Rudolf
Michlmayr Herbert
Schmeitzl Clemens
Schuhmacher Rainer
Warth Benedikt
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