Selected Publication:

Wilson, IBH; Zeleny, R; Kolarich, D; Staudacher, E; Stroop, CJ; Kamerling, JP; Altmann, F.
(2001): Analysis of Asn-linked glycans from vegetable foodstuffs: widespread occurrence of Lewis a, core alpha1,3-linked fucose and xylose substitutions
Glycobiology. 2001; 11(4):261-274 FullText FullText_BOKU

Abstract:

The N-glycans from 27 "plant" foodstuffs, including one from a gymnospermic plant and one from a fungus, were prepared by a new procedure and examined by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). For several samples, glycan structures were additionally investigated by size-fractionation and revel se-phase high-performance liquid chromatography in conjunction,vith exoglycosidase digests and finally also H-1-nuclear magnetic resonance spectroscopy. The glycans found ranged from the typical vacuolar "horseradish peroxidase" type and oligomannose to complex Le(a)-carrying structures. Though the common mushroom exclusively contained N-glycans of the oligomannosidic type, all plant foods contained mixtures of the above-mentioned types. Apple, asparagus, avocado, banana, carrot, celery, hazelnut, kiwi, onion, orange, pear, pignoli, strawberry, and walnut were particularly rich in Le(a)-carrying N-glycans. Although traces of Le(a)-containing structures were also present in almond, pistachio, potato, and tomato, no such glycans could be found in cauliflower. Coconut exhibited almost exclusively N-glyclans containing only xylose but no fucose, Oligomannosidic N-glycans dominated in buckwheat and especially in the legume seeds mung bean, pea, peanut, and soybean. Papaya presented a unique set of hybrid type structures partially containing the Le(a) determinant. These results are not only compatible with the hypothesis that the carbohydrate structures are another potential source of immunological cross-reaction between different plant allergens, but they also demonstrate that the Le(a)-type structure is very widespread among plants.

Authors BOKU Wien:

Altmann Friedrich

Staudacher Erika

Wilson Iain B.H.

Find related publications in this database (using NML MeSH Indexing)

Asparagine - metabolism

Carbohydrate Conformation - metabolism

Carbohydrate Sequence - metabolism

Chromatography, Gel - metabolism

Chromatography, High Pressure Liquid - metabolism

Epitopes - analysis

Food Hypersensitivity - analysis

Fruit - chemistry

Fucose - analogs & derivatives

Fungi - chemistry

Galactose - analysis

Glycoside Hydrolases - metabolism

Lewis Blood-Group System - analysis

Magnetic Resonance Spectroscopy - analysis

Molecular Sequence Data - analysis

Molecular Weight - analysis

Oligosaccharides - analysis

Polysaccharides - analysis

Seeds - chemistry

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - chemistry

Vegetables - chemistry

Xylose - analysis

Find related publications in this database (Keywords)

allergy

MALDI-TOF-MS

Lewis a

plant glycoproteins

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