Selected Publication:

Altman, E; Schäffer, C; Brisson, JR; Messner, P.
(1995): Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71.
Eur J Biochem. 1995; 229(1):308-315 FullText FullText_BOKU

Abstract:

The squarely arranged surface layer (S-layer) glycoprotein of Clostridium thermosaccharolyticum E207-71 was isolated from bacterial cells which were grown under defined culture conditions. By sodium dodecyl sulfate polyacrylamide gel electrophoresis, the S-layer showed a series of distinct bands with apparent molecular masses in the range 83-210 kDa. Upon deglycosylation by trifluoromethanesulfonic acid, only the single band at 83 kDa remained unchanged. After pronase digestion of the intact S-layer glycoprotein, the degradation products were isolated by gel-permeation chromatography, cation-exchange chromatography and isoelectric focusing. Three main fractions and an amino sugar containing minor fraction were obtained. The main fractions, which showed identical carbohydrate compositions, were further purified by reverse-phase chromatography and characterized by monosaccharide analysis, Smith degradation, methylation analysis, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. The combined chemical and spectroscopical evidence suggest the following glycan structure for the main fractions: [GRAPHICS] alNAc alpha substituted by Glc beta at O3; these differences are responsible for the only weak serological cross-reactivity of the two strains.

Authors BOKU Wien:

Messner Paul

Schäffer Christina

Find related publications in this database (using NML MeSH Indexing)

Amino Acid Sequence -

Carbohydrate Sequence -

Clostridium - chemistry

Magnetic Resonance Spectroscopy -

Membrane Glycoproteins - chemistry

Molecular Sequence Data -

Molecular Structure -

Polysaccharides - chemistry

Find related publications in this database (Keywords)

BACTERIA

EUBACTERIA

SURFACE LAYER

CRYSTALLINE BACTERIAL CELL SURFACE LAYER

GLYCOPROTEIN

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