University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Selected Publication:

Zámocký, M; Sekot, G; Bučková, M; Godočíková, J; Schäffer, C; Farkašovský, M; Obinger, C; Polek, B; .
(2013): Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s).
Arch Microbiol. 2013; 195(6):393-402 FullText FullText_BOKU

Bifunctional catalase-peroxidases (KatGs) are heme oxidoreductases widely spread among bacteria, archaea and among lower eukaryotes. In fungi, two KatG groups with different localization have evolved, intracellular (KatG1) and extracellular (KatG2) proteins. Here, the cloning, expression analysis and subcellular localization of two novel katG1 genes from the soil fungi Chaetomium globosum and Chaetomium cochliodes are reported. Whereas, the metalloenzyme from Ch. globosum is expressed constitutively, Ch. cochliodes KatG1 reveals a slight increase in expression after induction of oxidative stress by cadmium ions and hydrogen peroxide. The intronless open reading frames of both Sordariomycetes katG1 genes as well as of almost all fungal katG1s possess two peroxisomal targeting signals (PTS1 and PTS2). Peroxisomal localization of intracellular eukaryotic catalase-peroxidases was verified by organelle separation and immunofluorescence microscopy. Co-localization with the peroxisomal enzyme 3-ketoacyl-CoA-thiolase was demonstrated for KatGs from Magnaporthe grisea, Chaetomium globosum and Chaetomium cochliodes. The physiological role of fungal catalase-peroxidases is discussed.
Authors BOKU Wien:
Obinger Christian
Schäffer Christina
Zamocky Marcel
Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence;Chaetomium/cytology;Chaetomium/enzymology*;Hydrogen Peroxide/metabolism;Magnaporthe/enzymology;Molecular Sequence Data;Oxidative Stress;Peroxidases/chemistry;Peroxidases/genetics*;Peroxidases/metabolism;Sequence Alignment;Soil Microbiology*;

Find related publications in this database (Keywords)
Catalase-peroxidase (KatG)
Oxidative stress
Peroxisomal targeting
Real-time PCR

© BOKU Wien Imprint