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Selected Publication:

Varga, E; Wiesenberger, G; Fruhmann, P; Malachov√°, A; Svoboda, T; Lemmens, M; Adam, G; Berthiller, F; .
(2016): Pentahydroxyscirpene-Producing Strains, Formation In Planta, and Natural Occurrence.
Toxins (Basel). 2016; 8(10): FullText FullText_BOKU

Abstract:
Trichothecenes are a class of structurally diverse mycotoxins with more than 200 naturally occurring compounds. Previously, a new compound, pentahydroxyscirpene (PHS), was reported as a byproduct of a nivalenol producing Fusarium strain, IFA189. PHS contains a hydroxy group at C-8 instead of the keto group of type B trichothecenes. In this work, we demonstrate that IFA189 belongs to the species Fusarium kyushuense using molecular tools. Production of PHS in vitro was also observed for several isolates of other Fusarium species producing nivalenol. Furthermore, we report the formation of 4-acetyl-PHS by F. kyushuense on inoculated rice. Wheat ears of the variety Remus were infected with IFA189 and the in planta production of PHS was confirmed. Natural occurrence of PHS was verified in barley samples from the Czech Republic using a liquid chromatographic-tandem mass spectrometric method validated for this purpose. Toxicity of PHS to wheat ribosomes was evaluated with a coupled in vitro transcription and translation assay, which showed that PHS inhibits protein biosynthesis slightly less than nivalenol and deoxynivalenol.
Authors BOKU Wien:
Adam Gerhard
Berthiller Franz
Lemmens Marc
Malachova Alexandra
Svoboda Thomas
Varga Elisabeth
Wiesenberger Gerlinde
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
DNA, Fungal/analysis;Food Contamination/analysis;Fusarium/genetics;Fusarium/metabolism*;Hordeum/chemistry;Hordeum/microbiology;Oryza/chemistry;Oryza/microbiology*;Ribosomes/drug effects;Trichothecenes/metabolism*;Trichothecenes/toxicity;Triticum/chemistry;Triticum/microbiology*;

Find related publications in this database (Keywords)
mycotoxins
LC-MS/MS
taxonomy
toxicity
cereals


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