University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Selected Publication:

Gal, N; Lassenberger, A; Herrero-Nogareda, L; Scheberl, A; Charwat, V; Kasper, C; Reimhult, E.
(2017): Interaction of Size-Tailored PEGylated Iron Oxide Nanoparticles with Lipid Membranes and Cells
ACS BIOMATER SCI ENG. 2017; 3(3): 249-259. FullText FullText_BOKU

Targeted nanomedicine builds on the concept that nanoparticles can be directed to specific tissues while remaining inert to others organs. Many studies have been performed on the synthesis and cellular interactions of core-shell nanoparticles, in which a functional inorganic core is coated with a biocompatible polymer layer that should reduce nonspecific uptake and cytotoxicity. However, work is lacking that relates structural parameters of the core-shell structure and colloidal properties directly to interactions with cell membranes and further correlates these interactions to cell uptake. We have synthesized monodisperse (SD < 10%), single-crystalline, and superparamagnetic iron oxide nanoparticles (SPION) of different core size (3-8 nm) that are densely grafted with nitrodopamine-poly(ethylene glycol) (NDA-PEG(5 kDa)) brushes. We investigated the interactions of the PEGylated SPION with biomimetic membranes and cancer and kidney cells. It is shown that a dense homogeneous PEG shell suppresses membrane interactions and cell uptake but that nanoparticle curvature can influence membrane interactions for similarly grafted nanoparticles. Weak adsorption to anionic lipid membranes is shown to correlate with eukaryote cell uptake and is attributed to double-layer interactions without direct membrane penetration. This attraction is strongly suppressed during physiological conditions and leads to unprecedented low cell uptake and full cell viability when compared to those of traditional dextran-coated SPION. Less curved (larger core) PEGylated SPION show weaker membrane adsorption and lower cell uptake due to effectively denser shells. These results provide a better understanding of design criteria for core shell nanoparticles in terms of avoiding nonspecific uptake by cells, reducing toxicity, and increasing circulation time.
Authors BOKU Wien:
Almeria Ciarra
Charwat Verena
Gal Noga
Kasper Cornelia
Lassenberger Andrea
Reimhult Erik
Scheberl Andrea
BOKU Gendermonitor:

Find related publications in this database (Keywords)
superparamagnetic core-shell iron oxide PEG nanoparticles
supported bilayer
membrane interaction
cell uptake

© BOKU Wien Imprint