University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Iturri, J; Weber, A; Moreno-Cencerrado, A; Vivanco, MD; Benitez, R; Leporatti, S; Toca-Herrera, JL.
(2019):
Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy
INT J MOL SCI. 2019; 20(13), 3275
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- Abstract:
- Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 mu M) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young's modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0-6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment.
- Authors BOKU Wien:
- Iturri Jagoba
- Moreno Cencerrado Alberto
- Toca-Herrera José Luis
- Weber Andreas
- Find related publications in this database (Keywords)
- resveratrol
- MCF-7
- cytomechanics
- atomic force microscopy
- force spectroscopy
- fluorescence microscopy
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