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Selected Publication:

Iturri, J; Weber, A; Moreno-Cencerrado, A; Vivanco, MD; Benitez, R; Leporatti, S; Toca-Herrera, JL.
(2019): Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy
INT J MOL SCI. 2019; 20(13), 3275 FullText FullText_BOKU

Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 mu M) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young's modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0-6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment.
Authors BOKU Wien:
Iturri Jagoba
Moreno Cencerrado Alberto
Toca-Herrera José Luis
Weber Andreas

Find related publications in this database (Keywords)
atomic force microscopy
force spectroscopy
fluorescence microscopy

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