University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Hohenwarter, O; Jakoubek, A; Schmatz, C; Katinger, H.
(1994):
Expression of SV40 tumour antigens enables human endothelial cells to grow independently from foetal calf serum and exogenous growth factors.
J Biotechnol. 1994; 34(2):205-211
FullText
FullText_BOKU
- Abstract:
- Human endothelial cells were transfected with a plasmid containing the coding sequence of the large T protein of simian virus 40. Transfected cells were selected for their ability to grow in defined medium (DM). Several cell lines were derived and characterized in their response to endothelial cell growth supplement (ECGS), epidermal growth factor (EGF) and insulin (INS). In addition to cell lines that were dependent on these additives, others growing without any exogenous growth factor could be selected. No evidence of autocrine growth stimulation was found. For growth studies, a simple assay was used based on the acid phosphatase activity as a parameter for the cell number. Cell lines in defined medium showed less chromosome aberrations than those grown in serum-containing medium. Because of their long in vitro life span of about 100 generation doublings and defined medium requirements these cells represent valuable test material for all kinds of investigations on the vascular endothelium.
- Authors BOKU Wien:
- Katinger Hermann
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Antigens, Polyomavirus Transforming - biosynthesis
- Blood - biosynthesis
- Cattle - biosynthesis
- Cell Division - drug effects
- Cells, Cultured - drug effects
- Culture Techniques - methods
- Endothelium, Vascular - cytology
- Growth Substances - pharmacology
- Humans - pharmacology
- Kinetics - pharmacology
- Plasmids - pharmacology
- Simian virus 40 - genetics
- Transfection - genetics
- Umbilical Veins - genetics
- Find related publications in this database (Keywords)
- HUMAN ENDOTHELIAL CELL
- SV40
- ONCOGENE
- GROWTH FACTOR
- DEFINED MEDIUM
- CELL PROPAGATION
- ACID PHOSPHATASE
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