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Selected Publication:

Pachlinger, R; Mitterbauer, R; Adam, G; Strauss, J.
(2005): Metabolically independent and accurately adjustable Aspergillus sp. expression system.
Appl Environ Microbiol. 2005; 71(2):672-678 FullText FullText_BOKU

Filamentous fungi are well-established expression hosts often used to produce extracellular proteins of use in the food and pharmaceutical industries. The expression systems presently used in Aspergilhis species rely on either strong constitutive promoters, e.g., that for glyceraldehyde-3-phosphate dehydrogenase, or inducible systems derived from metabolic pathways, e.g., gla-4 (glucoamylase) or ale (alcohol dehydrogenase). We describe for Aspergillus nidulans and Aspergillus niger a novel expression system that utilizes the transcriptional activation of the human estrogen receptor by estrogenic substances. The system functions independently from metabolic signals and therefore can be used with low-cost, complex media. A combination of positive and negative regulatory elements in the promoter drives the expression of a reporter gene, yielding a linear dose response to the inducer. The off status is completely tight, yet the system responds within minutes to induction and reaches a level of expression of up to 15% of total cell protein after 8 h. Both Aspergillus species are very sensitive to estrogenic substances, and low-cost inducers function in the picomolar concentration range, at which estrogenic substances also can be found in the environment. Given this high sensitivity to estrogens, Aspergillus cells carrying estrogen-responsive units could be used to detect xenoestrogens in food or in the environment.
Authors BOKU Wien:
Adam Gerhard
Strauss Joseph
Find related publications in this database (using NML MeSH Indexing)
Aspergillus nidulans - genetics
Aspergillus niger - genetics
Biotechnology - methods
Culture Media - methods
Diethylstilbestrol - metabolism
Estrogen Receptor alpha - genetics
Estrogens - analysis
Gene Expression Regulation, Fungal - analysis
Genes, Reporter - analysis
Glucosephosphate Dehydrogenase - analysis
Humans - analysis
Promoter Regions, Genetic - analysis
beta-Galactosidase - genetics

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