University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Selected Publication:

Li, A; Baba, TW; Sodroski, J; Zolla-Pazner, S; Gorny, MK; Robinson, J; Posner, MR; Katinger, H; Barbas, CF; Burton, DR; Chou, TC; Ruprecht, RM.
(1997): Synergistic neutralization of a chimeric SIV/HIV type 1 virus with combinations of human anti-HIV type 1 envelope monoclonal antibodies or hyperimmune globulins.
AIDS Res Hum Retroviruses. 1997; 13(8):647-656

A panel of 14 human IgG monoclonal antibodies (MAbs) specific for envelope antigens of the human immunodeficiency virus type 1 (HIV-1), 2 high-titer human anti-HIV-1 immunoglobulin (HIVIG) preparations, and 15 combinations of MAbs or MAb/HIVIG were tested for their ability to neutralize infection of cultured human T cells (MT-2) with a chimeric simian immunodeficiency virus (SHIV-vpu(+)), which expressed HIV-1 IIIB envelope antigens, Eleven MAbs and both HIVIGs were neutralizing, When used alone, the anti-CD4-binding site MAb b12, the anti-gp41 MAb 2F5, and the anti-gp120 MAb 2G12 were the most potent, When combination regimens involving two MAbs targeting different epitopes were tested, synergy was seen in all paired MAbs, except for one combination that revealed additive effects, The lowest effective antibody concentration for 50% viral neutralization (EC50) and EC90 were achieved with combinations of MAbs b12, 2F5, 2G12, and the anti-V3 MAb 694/98D, Depending on the combination regimen, the concentration of MAbs required to reach 90% virus neutralization was reduced approximately 2- to 25-fold as compared to the dose requirement of individual MAbs to produce the same effect, Synergy of the combination regimens implies that combinations of antibodies may have a role in passive immunoprophylaxis against HIV-1, The ability of SHIV to replicate in rhesus macaques will allow us to test such approaches in vivo.
Authors BOKU Wien:
Katinger Hermann
Find related publications in this database (using NML MeSH Indexing)
Animals -
Antibodies, Monoclonal - immunology
Antigens, Viral - immunology
Cells, Cultured -
Chimera - drug effects
Drug Synergism -
HIV Antibodies - immunology
HIV-1 - drug effects
Human Immunodeficiency Virus Proteins -
Humans -
Immunoglobulins - immunology
Macaca mulatta -
Neutralization Tests -
Simian immunodeficiency virus - drug effects
Viral Envelope Proteins - immunology
Viral Regulatory and Accessory Proteins - drug effects

© BOKU Wien Imprint