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Marzban, G; Grillari, J; Reisinger, E; Hemetsberger, T; Grabherr, R; Katinger, H; .
(2002): Age-related alterations in the protein expression profile of C57BL/6J mouse pituitaries.
Exp Gerontol. 2002; 37(12):1451-1460

Abstract:: The aim of our study was to monitor the protein expression profile in pituitary glands of healthy C57BL/6J mice during aging. Pituitary glands of 4-week old (immature), 3-month old (mature), and > 25-month old mice were analysed by proteomic tools such as two-dimensional electrophoresis and N-terminal micro-sequencing. A change was detected in the expression of growth hormone after sexual maturation. Our particular interest, however, was directed against up-regulated proteins in the old pituitary glands, which are proposed to be involved in the process of neuroendocrine aging. Among these proteins, the expression of glutathione-S-transferase (GST) and apolipoprotein A-1 were increased in old pituitaries. Furthermore, ubiquitin carboxyl-terminal hydrolase (UCH-L1) was significantly up-regulated in senescent C57BL/6J mouse pituitaries. Since only the rat homologue was known, we isolated and analysed the mouse UCH-L1 sequence. Since GST is involved in antioxidative defence and UCH-L1 is part of the ubiquitin/proteasome system, which is responsible for the removal of damaged proteins, these results suggest increased oxidative burden and an increased activity of the ubiquitin system. (C) 2002 Elsevier Science Inc. All rights reserved.
Authors BOKU Wien:: Grabherr Reingard; Grillari Johannes; Katinger Hermann; Marzban Gorji
Find related publications in this database (using NML MeSH Indexing): Aging/genetics;Aging/metabolism*;Amino Acid Sequence;Animals;Apolipoprotein A-I/metabolism;Electrophoresis, Gel, Two-Dimensional;Gene Expression Profiling;Glutathione Transferase/metabolism;Mice;Mice, Inbred C57BL;Mice, Inbred Strains;Molecular Sequence Data;Pituitary Gland/metabolism*;Proteins/genetics;Proteins/metabolism*;Thiolester Hydrolases/metabolism;Ubiquitin Thiolesterase;Up-Regulation/physiology;
Find related publications in this database (Keywords): pituitary gland; aging; ubiquitin carboxyterminal-hydrolase (UCH-L1); two-dimensional electrophoresis