University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Puehringer, HM; Zinoecker, I; Marzban, G; Katinger, H; Laimer, M.
(2003):
MdAP, a novel protein in apple, is associated with the major allergen Mal d 1.
Gene. 2003; 321(1):173-183
- Abstract:
- Mal d 1, an 18-kDa intracellular pathogenesis-related protein (PR-10), has been known since long as the major apple allergen in Middle and Northern Europe. However, its biological function, as that of many other PR-10 proteins, is still unknown. In order to identify proteins putatively interacting with Mal d 1, an expression library of Malus domestica was screened using the yeast-two-hybrid (Y2H) system. A novel protein binding to two isoforms of Mal d 1 being used as 'bait' was isolated. The deduced amino acid sequence from the corresponding full-length cDNA of the predicted Mal d 1-Associated-Protein (MdAP) do not display any homology to known proteins, but shares 45% identity with a 'hypothetical protein' in Arabidopsis thaliana. Southern analysis of the apple genome indicated that MdAP, comprising 190 amino acids, is encoded by a single gene. The expression pattern of the 1-kb MdAP transcript resembled the expression profile of the different Mal d 1 isoforms in various apple organs, however at a much lower level. Furthermore, a huge variation in transcription levels of Mal d 1 isoforms was observed in apple tissue. For both, Mal d I and MdAP highest amounts of mRNAs were measured in ripe fruits and significantly lower amounts in vegetative tissue by quantitative reverse transcription-polymerase chain reaction (RT-PCR). (C) 2003 Elsevier B.V. All fights reserved.
- Authors BOKU Wien:
- Katinger Hermann
- Laimer Margit
- Marzban Gorji
- Find related publications in this database (using NML MeSH Indexing)
- Allergens - genetics
- Amino Acid Sequence -
- Arabidopsis Proteins - genetics
- Base Sequence -
- Blotting, Northern -
- DNA, Plant - chemistry
- Gene Expression Regulation, Plant -
- Malus - genetics
- Molecular Sequence Data -
- Phylogeny -
- Plant Proteins - genetics
- Protein Binding -
- RNA, Messenger - genetics
- Saccharomyces cerevisiae - genetics
- Sequence Alignment -
- Sequence Analysis, DNA -
- Sequence Homology, Amino Acid -
- Two-Hybrid System Techniques -
- Find related publications in this database (Keywords)
- Malus domestica
- mal d 1-associated-protein
- yeast-two-hybrid-system
- pathogenesis-related protein
- PR-10
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