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Selected Publication:

Ferner-Ortner, J; Mader, C; Ilk, N; Sleytr, UB; Egelseer, EM.
(2007): High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology.
J Bacteriol. 2007; 189(19):7154-7158 FullText FullText_BOKU

Surface plasmon resonance studies using C-terminal truncation forms of the S-layer protein SbsC (recombinant SbsC consisting of amino acids 31 to 270 [rSbSC(31-270)] and rSbsC(31-443)) and the secondary cell wall polymer (SCWP) isolated from Geobacillus stearothermophilus ATCC 12980 confirmed the exclusive responsibility of the N-terminal region comprising amino acids 31 to 270 for SC"T binding. Quantitative analyses indicated binding behavior demonstrating low, medium, and high affinities.
Authors BOKU Wien:
Egelseer Eva Maria
Sleytr Uwe B.
Find related publications in this database (using NML MeSH Indexing)
Bacillus stearothermophilus - metabolism
Bacterial Proteins - chemistry
Cell Wall - metabolism
Electrophoresis, Polyacrylamide Gel - metabolism
Kinetics - metabolism
Peptide Fragments - chemistry
Protein Binding - chemistry
Surface Plasmon Resonance - methods

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