University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Ferner-Ortner, J; Mader, C; Ilk, N; Sleytr, UB; Egelseer, EM.
(2007):
High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology.
J Bacteriol. 2007; 189(19):7154-7158
FullText
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- Abstract:
- Surface plasmon resonance studies using C-terminal truncation forms of the S-layer protein SbsC (recombinant SbsC consisting of amino acids 31 to 270 [rSbSC(31-270)] and rSbsC(31-443)) and the secondary cell wall polymer (SCWP) isolated from Geobacillus stearothermophilus ATCC 12980 confirmed the exclusive responsibility of the N-terminal region comprising amino acids 31 to 270 for SC"T binding. Quantitative analyses indicated binding behavior demonstrating low, medium, and high affinities.
- Authors BOKU Wien:
- Egelseer Eva Maria
- Sleytr Uwe B.
- Find related publications in this database (using NML MeSH Indexing)
- Bacillus stearothermophilus - metabolism
- Bacterial Proteins - chemistry
- Cell Wall - metabolism
- Electrophoresis, Polyacrylamide Gel - metabolism
- Kinetics - metabolism
- Peptide Fragments - chemistry
- Protein Binding - chemistry
- Surface Plasmon Resonance - methods
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