University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Gach, JS; Quendler, H; Strobach, S; Katinger, H; Kunert, R.
(2008):
Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5.
Mol Immunol. 2008; 45(4):1027-1034
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- Abstract:
- Anti-idiotypic antibody (Ab2) 3H6 is directed against the human monoclonal antibody 2175, which is one of a few neutralising antibodies against HIV-1. Since the binding epitope of 2175 is cryptic and no neutralising immune response could be elicited by several potential vaccines comprising this region, Ab2/3H6 represents a potent vaccine candidate for active immunisation. Here we describe the molecular features of Ab2/3H6 after changing the antigen binding specificity by single point mutations in the complementarity-determining region 3 of the Ab2/3H6 heavy chain. The resulting Ab2/3H6 mutants were compared in several experimental settings to the wild type Ab2/3H6 Fab fragment. Moreover, we report about an immunisation study with Ab2/3H6 Fab variants, which elicited a specific 2F5-like humoral immune response in BALB/c mice. (c) 2007 Elsevier Ltd. All rights reserved.
- Authors BOKU Wien:
- Katinger Hermann
- Kunert Renate
- Quendler Heribert
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Antibodies, Anti-Idiotypic - administration & dosage
- Antibodies, Monoclonal - immunology
- Antibody Formation -
- CHO Cells -
- Complementarity Determining Regions - administration & dosage
- Cricetinae -
- Cricetulus -
- Epitopes -
- Female -
- Humans -
- Immunoglobulin Fab Fragments - administration & dosage
- Immunoglobulin Heavy Chains - administration & dosage
- Mice -
- Mice, Inbred BALB C -
- Point Mutation -
- Find related publications in this database (Keywords)
- 2F5
- HIV-1
- anti-idiotypic antibodies
- vaccine
- ELDKWA
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