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Selected Publication:

van Hoek, AH; Mayrhofer, S; Domig, KJ; Flórez, AB; Ammor, MS; Mayo, B; Aarts, HJ.
(2008): Mosaic tetracycline resistance genes and their flanking regions in Bifidobacterium thermophilum and Lactobacillus johnsonii.
Antimicrob Agents Chemother. 2008; 52(1):248-252 FullText FullText_BOKU

Abstract:
For the first time, mosaic tetracycline resistance genes were identified in Lactobacillus johnsonii and in Bifidobacterium thermophilum strains. The L. johnsonii strain investigated contains a complex hybrid gene, tet(O/W/32/O/W/O), whereas the five bifidobacterial strains possess two different mosaic tet genes: i.e., tet(W/ 32/O) and tet(O/W). As reported by others, the crossover points of the mosaic tet gene segments were found at similar positions within the genes, suggesting a hot spot for recombination. Analysis of the sequences flanking these genes revealed that the upstream part corresponds to the 5' end of the mosaic open reading frame. In contrast, the downstream region was shown to be more variable. Surprisingly, in one of the B. thermophilum strains a third let determinant was identified, coding for the efflux pump Tet(L).
Authors BOKU Wien:
Domig Konrad
Mayrhofer Sigrid
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Bacterial Proteins - genetics
Bifidobacterium - classification
Carrier Proteins - genetics
DNA, Bacterial - analysis
Feces - microbiology
Humans -
Lactobacillus - classification
Molecular Sequence Data -
Polymerase Chain Reaction -
Polymorphism, Restriction Fragment Length -
Recombination, Genetic -
Sequence Analysis, DNA -
Swine -
Tetracycline Resistance - genetics



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