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Kainz, B; Steiner, K; Möller, M; Pum, D; Schäffer, C; Sleytr, UB; Toca-Herrera, JL.
(2010): Absorption, steady-state fluorescence, fluorescence lifetime, and 2D self-assembly properties of engineered fluorescent S-layer fusion proteins of Geobacillus stearothermophilus NRS 2004/3a.
Biomacromolecules. 2010; 11(1):207-214 FullText FullText_BOKU

Abstract:: S-layer fusion protein technology was used to design four different fluorescent fusion proteins with three different GFP mutants and the red fluorescent protein mRFP1. Their absorption spectra, steady-state fluorescence, and fluorescence lifetime were investigated as a function of pH. It was found that fluorescence intensities and lifetime of the GFP mutant S-layer fusion proteins decreased about 50% between pH 6 and pH 5. The spectral properties of the red S-layer fusion protein were minimally affected by pH variations. These results were compared with His-tagged reference fluorescent proteins, demonstrating that the S-layer protein did not change the general spectral properties of the whole fusion protein. In addition, the pK(a) values of the fluorescent S-layer fusion proteins were calculated. Finally, it was shown that the S-layer fusion proteins were able to self-assemble forming 2D nanostructures of oblique p2 symmetry with lattice parameters of about a = 11 nm, b = 14 nm, and gamma = 80 degrees. The fluorescence tag did not hinder the natural self-assembly process of the S-layer protein. The combination of the fluorescence properties and the self-assembly ability of the engineered fusion proteins make them a promising tool to generate biomimetic surfaces for future applications in nanobiotechnology at a wide range of pH.
Authors BOKU Wien:: Pum Dietmar; Schäffer Christina; Sleytr Uwe B.; Toca-Herrera José Luis
Find related publications in this database (using NML MeSH Indexing): Absorption -; Bacterial Proteins - chemistry; Fluorescence -; Geobacillus stearothermophilus - enzymology; Green Fluorescent Proteins - chemistry; Protein Engineering -; Recombinant Fusion Proteins - chemistry