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Selected Publication:

Liebminger, E; Hüttner, S; Vavra, U; Fischl, R; Schoberer, J; Grass, J; Blaukopf, C; Seifert, GJ; Altmann, F; Mach, L; Strasser, R; .
(2009): Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana.
Plant Cell. 2009; 21(12):3850-3867 FullText FullText_BOKU

Abstract:
In eukaryotes, class I alpha-mannosidases are involved in early N-glycan processing reactions and in N-glycan-dependent quality control in the endoplasmic reticulum (ER). To investigate the role of these enzymes in plants, we identified the ER-type alpha-mannosidase I (MNS3) and the two Golgi-alpha-mannosidase I proteins (MNS1 and MNS2) from Arabidopsis thaliana. All three MNS proteins were found to localize in punctate mobile structures reminiscent of Golgi bodies. Recombinant forms of the MNS proteins were able to process oligomannosidic N-glycans. While MNS3 efficiently cleaved off one selected alpha 1,2-mannose residue from Man(9)GlcNAc(2), MNS1/2 readily removed three alpha 1,2-mannose residues from Man(8)GlcNAc(2). Mutation in the MNS genes resulted in the formation of aberrant N-glycans in the mns3 single mutant and Man(8)GlcNAc(2) accumulation in the mns1 mns2 double mutant. N-glycan analysis in the mns triple mutant revealed the almost exclusive presence of Man(9)GlcNAc(2), demonstrating that these three MNS proteins play a key role in N-glycan processing. The mns triple mutants displayed short, radially swollen roots and altered cell walls. Pharmacological inhibition of class I alpha-mannosidases in wild-type seedlings resulted in a similar root phenotype. These findings show that class I alpha-mannosidases are essential for early N-glycan processing and play a role in root development and cell wall biosynthesis in Arabidopsis.
Authors BOKU Wien:
Altmann Friedrich
Blaukopf Claudia
Fischl Richard
Grass Josephine
Hüttner Silvia
Liebminger Eva
Mach Lukas
Schoberer Jennifer
Seifert Georg
Strasser Richard
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Find related publications in this database (using NML MeSH Indexing)
Animals
Arabidopsis/enzymology*
Arabidopsis/genetics
Arabidopsis Proteins/genetics
Arabidopsis Proteins/metabolism*
Cell Line
Cell Wall/metabolism
Cloning, Molecular
DNA, Bacterial/genetics
Endoplasmic Reticulum/enzymology
Genetic Complementation Test
Glycosylation
Golgi Apparatus/enzymology
Mutagenesis, Insertional
Mutation
Phylogeny
Plant Roots/enzymology
Plant Roots/growth & development*
Polysaccharides/metabolism*
RNA, Plant/genetics
Spodoptera/cytology
Substrate Specificity
alpha-Mannosidase/genetics
alpha-Mannosidase/metabolism*



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