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Selected Publication:

Song, H; Ritz, S; Knoll, W; Sinner, EK.
(2009): Conformation and topology of amyloid beta-protein adsorbed on a tethered artificial membrane probed by surface plasmon field-enhanced fluorescence spectroscopy.
J Struct Biol. 2009; 168(1):117-124 FullText FullText_BOKU

Abstract:
Progressive depositions of cerebral amyloid are primary neuropathologic features of Alzheimer's disease (AD). The amyloid is composed of a 39-42 amino acid peptide called the amyloid beta-protein (A beta). Repeated investigation suggests that the conformational transition of A beta from a-helix or random coil to beta-sheet structure plays a key role in the inappropriate accumulation of cerebral amyloid plaques. In this manuscript, we describe a fluorescence-based immunoassay technology to investigate the conformation and topology of A beta peptides interacting with peptide-tethered planar lipid bilayers. Dual monoclonal antibodies (mAbs) labelled with fluorophores were employed to recognise a linear N- and a beta-sheet C-terminus of A beta peptides on the model membrane, respectively. Kinetics of antibody-A beta binding were determined by surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The conformational transition of A beta by melatonin, a defined beta-sheet breaker, was probed using paired monoclonal antibodies. The A beta interaction with the membrane was evaluated by carefully analyzing the change in kinetic/affinity parameters in the presence or absence of melatonin. These results show that SPFS can be used to examine conformational transition of A beta on an artificial membrane, providing a novel and versatile platform for conveniently monitoring protein-membrane interaction and screening for new beta-sheet breakers. (C) 2009 Elsevier Inc. All rights reserved.
Authors BOKU Wien:
Ehmoser Eva-Kathrin
Find related publications in this database (using NML MeSH Indexing)
Absorption -
Amyloid beta-Peptides - chemistry
Lipid Bilayers - chemistry
Membranes, Artificial -
Models, Biological -
Spectrometry, Fluorescence -
Surface Plasmon Resonance -

Find related publications in this database (Keywords)
Alzheimerxxxs disease (AD)
Amyloid beta peptide (A beta)
Immunoassay
Melatonin
SPS
SPFS


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