University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Selected Publication:

Wiltschi, B; Schober, M; Kohlwein, SD; Oesterhelt, D; Sinner, EK.
(2006): Sterol binding assay using surface plasmon fluorescence spectroscopy.
Anal Chem. 2006; 78(2):547-555 FullText FullText_BOKU

Abstract:
We describe the design of a novel in vitro assay to study the interaction of soluble proteins with small hydrophobic sterol ligands. The sterol molecules are incorporated in an artificial membrane system in order to mimic their arrangement found in a biomembrane. The artificial membrane setup is monitored in real time by surface plasmon spectroscopy. Binding of fluorescently labeled soluble protein is observed by optical detection with surface plasmon enhanced fluorescence spectroscopy. By application of the novel assay, we demonstrate that four different oxidized sterol molecules are specifically recognized by the yeast protein Osh5p, a presumed oxysterol binding protein. Osh5p from yeast is the first oxysterol binding protein homologue for which oxysterol binding is shown with this new technique. With the design of our novel in vitro oxysterol binding assay, we have solved the technically challenging difficulty of presenting hydrophobic ligands to hydrophilic proteins in aqueous media.
Authors BOKU Wien:
Ehmoser Eva-Kathrin
Wiltschi Birgit
Find related publications in this database (using NML MeSH Indexing)
Ergosterol - analogs & derivatives
Membranes, Artificial -
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Spectrometry, Fluorescence -
Sterols - analysis
Surface Plasmon Resonance -



Altmetric:
© BOKU Wien Imprint