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Oberlerchner, JT; Fuchs, C; Grausgruber, H; Potthast, A; Bohmdorfer, S.
(2018): A cote calibration - Making optimal use of time and space in quantitative high performance thin layer chromatography
J CHROMATOGR A. 2018; 1533: 193-198. FullText FullText_BOKU

Quantitative High Performance Thin Layer Chromatography (HPTLC) requires the application of several standards to each plate, reducing the number of actual samples that can be analyzed in a single run. Using pure standard compounds and a selective detection method, the standards for quantitation can be applied besides a cote the chromatography area. This frees the sample application space to accommodate the maximal number of sample on each plate. Also, analysis time is spent exclusively on samples, drastically shortening the effective analysis time per sample and increasing sample throughput. Using this new calibration approach, the sample capacity of regular HPTLC methods can be increased or their scope be extended by an additional quantitative analysis. As a limitation, changes to the distribution of samples and standards within the plate as well as interferences from matrix compounds must be observed. We demonstrate the feasibility of this method by complementing an HPTLC method with a quantitative analysis of total anthocyanin content in colored wheat varieties. The quantitation was validated and compared to the conventional photometric analysis. As outcome, the additional photometric analysis could be replaced and rendered unnecessary, saving time, effort and equipment. This approach could also be employed to quantify highly retained substances, which are usually inaccessible for quantitative analysis. (C) 2017 Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Böhmdorfer Stefan
Fuchs Christina
Grausgruber Heinrich
Oberlerchner Josua Timotheus
Potthast Antje

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