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Tegl, G; Stagl, V; Mensah, A; Huber, D; Somitsch, W; Grosse-Kracht, S; Guebitz, GM.
(2018): The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties
ENG LIFE SCI. 2018; 18(5): 334-340. FullText FullText_BOKU

Silicate-based microporous materials like zeolites are nano enabled particles and used for various applications including pharmaceutical formulations. This study reports on the chemo-enzymatic functionalization of chitosan-zeolite particles (CTS-zeolites) with caffeic acid (CA) and glucose oxidase (GOX) to impart combined antioxidant and antimicrobial properties. CA was grafted on the chitosan moieties by using laccase generating stable particles (zeta potential -36.7 mV) of high antioxidant activity (44% DPPH inhibition). GOX was immobilized both on CTS-zeolites and on CA modified CTS-zeolites and creating a hydrogen peroxide generation system continuously and in-situ producing this oxidative and antimicrobial agent. The system prevented bacterial growth of E. coli and S. aureus over 24 h whereby a steady-state concentration of around 60 mu M hydrogen peroxide in the culture medium was observed. CA and GOX functionalized CTS-zeolite particles additionally showed combinatorial antioxidant and antimicrobial properties providing a powerful bioactive system for medical applications. These particles proved their suitability for incorporation in bioactive formulations which could be used, inter alia, for topical wound treatments.
Authors BOKU Wien:
G├╝bitz Georg
Huber Daniela
Mensah Anna
Tegl Gregor Franz
BOKU Gendermonitor:

Find related publications in this database (Keywords)
Caffeic acid
Enzyme immobilization
Glucose oxidase
Hydrogen peroxide

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