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Selected Publication:

Altmann, F.
(1998): Structures of the N-linked carbohydrate of ascorbic acid oxidase from zucchini.
Glycoconj J. 1998; 15(1):79-82 FullText FullText_BOKU

The N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoproteins. To prevent misinterpretations of immunological data, the structure of the N-glycan of ascorbic acid oxidase has been reinvestigated. The oligosaccharides were released by almond N-glycosidase and analysed as their pyridylamino derivatives by 2D-HPLC and exoglycosidase digestions. The main structure resembled the typical complex plant N-glycan consisting of a core-pentasaccharide decorated with xylose and 3-linked fucose. The other abundant species lacked the fucose residue. Small amounts of these glycans carried a GlcNAc residue on the 6-arm. Therefore, ascorbic acid oxidase will not only react with antibodies directed against the xylosylated region but also with those binding to N-glycans with 3-linked fucose.
Authors BOKU Wien:
Altmann Friedrich
Find related publications in this database (using NML MeSH Indexing)
Ascorbate Oxidase - chemistry
Carbohydrate Sequence - chemistry
Cucurbitaceae - enzymology
Glycoproteins - chemistry
Molecular Sequence Data - chemistry
Plant Proteins - chemistry
Polysaccharides - chemistry

Find related publications in this database (Keywords)
asparagine-linked oligosaccharides
plant glycoproteins

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