University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Altmann, F.
(1998):
Structures of the N-linked carbohydrate of ascorbic acid oxidase from zucchini.
Glycoconj J. 1998; 15(1):79-82
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- Abstract:
- The N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoproteins. To prevent misinterpretations of immunological data, the structure of the N-glycan of ascorbic acid oxidase has been reinvestigated. The oligosaccharides were released by almond N-glycosidase and analysed as their pyridylamino derivatives by 2D-HPLC and exoglycosidase digestions. The main structure resembled the typical complex plant N-glycan consisting of a core-pentasaccharide decorated with xylose and 3-linked fucose. The other abundant species lacked the fucose residue. Small amounts of these glycans carried a GlcNAc residue on the 6-arm. Therefore, ascorbic acid oxidase will not only react with antibodies directed against the xylosylated region but also with those binding to N-glycans with 3-linked fucose.
- Authors BOKU Wien:
- Altmann Friedrich
- Find related publications in this database (using NML MeSH Indexing)
- Ascorbate Oxidase - chemistry
- Carbohydrate Sequence - chemistry
- Cucurbitaceae - enzymology
- Glycoproteins - chemistry
- Molecular Sequence Data - chemistry
- Plant Proteins - chemistry
- Polysaccharides - chemistry
- Find related publications in this database (Keywords)
- asparagine-linked oligosaccharides
- plant glycoproteins
- N-glycans
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