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Seidl, B; Rehak, K; Bueschl, C; Parich, A; Buathong, R; Wolf, B; Doppler, M; Mitterbauer, R; Adam, G; Khewkhom, N; Wiesenberger, G; Schuhmacher, R.
(2022): Gramiketides, Novel Polyketide Derivatives of Fusarium graminearum, Are Produced during the Infection of Wheat
J FUNGI. 2022; 8(10), 1030 FullText FullText_BOKU

Abstract:
The plant pathogen Fusarium graminearum is a proficient producer of mycotoxins and other in part still unknown secondary metabolites, some of which might act as virulence factors on wheat. The PKS15 gene is expressed only in planta, so far hampering the identification of an associated metabolite. Here we combined the activation of silent gene clusters by chromatin manipulation (kmt6) with blocking the metabolic flow into the competing biosynthesis of the two major mycotoxins deoxynivalenol and zearalenone. Using an untargeted metabolomics approach, two closely related metabolites were found in triple mutants (kmt6 tri5 pks4,13) deficient in production of the major mycotoxins deoxynivalenol and zearalenone, but not in strains with an additional deletion in PKS15 (kmt6 tri5 pks4,13 pks15). Characterization of the metabolites, by LC-HRMS/MS in combination with a stable isotope-assisted tracer approach, revealed that they are likely hybrid polyketides comprising a polyketide part consisting of malonate-derived acetate units and a structurally deviating part. We propose the names gramiketide A and B for the two metabolites. In a biological experiment, both gramiketides were formed during infection of wheat ears with wild-type but not with pks15 mutants. The formation of the two gramiketides during infection correlated with that of the well-known virulence factor deoxynivalenol, suggesting that they might play a role in virulence.
Authors BOKU Wien:
Adam Gerhard
B├╝schl Christoph
Doppler Maria
Parich Alexandra
Schuhmacher Rainer
Seidl Bernhard Klaus
Wiesenberger Gerlinde
Wolf Bernhard
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Find related publications in this database (Keywords)
Fusarium graminearum
Fusarium head blight
wheat
PKS15
polyketides
gramiketide
LC-HRMS
MS


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