University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Selected Publication:

Schuller, A; Studt-Reinhold, L; Strauss, J.
(2022): How to Completely Squeeze a Fungus-Advanced Genome Mining Tools for Novel Bioactive Substances
PHARMACEUTICS. 2022; 14(9), 1837 FullText FullText_BOKU

Fungal species have the capability of producing an overwhelming diversity of bioactive substances that can have beneficial but also detrimental effects on human health. These so-called secondary metabolites naturally serve as antimicrobial "weapon systems", signaling molecules or developmental effectors for fungi and hence are produced only under very specific environmental conditions or stages in their life cycle. However, as these complex conditions are difficult or even impossible to mimic in laboratory settings, only a small fraction of the true chemical diversity of fungi is known so far. This also implies that a large space for potentially new pharmaceuticals remains unexplored. We here present an overview on current developments in advanced methods that can be used to explore this chemical space. We focus on genetic and genomic methods, how to detect genes that harbor the blueprints for the production of these compounds (i.e., biosynthetic gene clusters, BGCs), and ways to activate these silent chromosomal regions. We provide an in-depth view of the chromatin-level regulation of BGCs and of the potential to use the CRISPR/Cas technology as an activation tool.
Authors BOKU Wien:
Schüller Andreas
Strauss Joseph
Studt-Reinhold Lena

Find related publications in this database (Keywords)
genome mining
secondary metabolism
BGC prediction
BGC activation
cluster activation
filamentous fungi

© BOKU Wien Imprint