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Sekandi, P; Namukobe, J; Byamukama, R; Nagawa, CB; Barbini, S; Bacher, M; Bohmdorfer, S; Rosenau, T.
(2023): Antimicrobial, antioxidant, and sun protection potential of the isolated compounds from Spermacoce princeae (K. Schum)
BMC COMPLEMENT MED. 2023; 23(1), 201 FullText FullText_BOKU

BackgroundSpermacoce princeae (K. Schum) has been used in the treatment of bacterial skin infections in Uganda. Pharmacological studies revealed that extracts of S. princeae exhibited antibacterial, antioxidant, and sun protection potential. This study aimed at isolating and identifying pure compounds from the extracts based on comprehensive analytical characterization by multiple analytical techniques.MethodsThe plant samples were extracted by sequential maceration using n-hexane, ethyl acetate, methanol, and distilled water. The compounds were isolated using a combination of chromatographic techniques and their structures were elucidated by multiple spectroscopic techniques. The antibacterial and antifungal activity determination of the isolated compounds was carried out using an agar well diffusion and potato dextrose assay against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Candida albicans, and Aspergillus flavus while the antioxidant activity was screened with the 2,2-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. The sun protection factor was determined using a Shimadzu Ultra Violet-visible (UV-VIS) double beam spectrophotometer between 290 to 320 nm.ResultsEleven compounds; quercetin (1), kaempferol-3-O-rutinoside (2), rutin (3, 12), myo-inositol (4), asperulosidic acid (5), hexadecanoic acid (6), & beta;-sitosterol (7), stigmasterol (8), campesterol (9), ursolic acid (10), and & beta;-sitosterol glucoside (11) were identified in the S. princeae extracts. Compound 2 had good antifungal activity against C. albicans (zone of inhibition, 23.0 & PLUSMN; 0.1 mm). Compound 10 showed antibacterial and antifungal activity against S. aureus, P. aeruginosa, C. albicans, and A. flavus. Compound 2 had a good percentage radical scavenging effect (IC50 = 64.81 & mu;g/ml) and a good sun protection factor (SPF = 26.83).ConclusionThis study reports the first-time isolation and identification of compounds 1 to 11 from S. princeae, which contribute to its antimicrobial, antioxidant, and sun protection potential.
Authors BOKU Wien:
Bacher Markus
Barbini Stefano
Böhmdorfer Stefan
Rosenau Thomas

Find related publications in this database (Keywords)
Skin infections
Spermacoce princeae
Antioxidant activity
Antimicrobial activity
Sun protection potential

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