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Selected Publication:

Graumann, K; Jungbauer, A.
(2000): Quantitative assessment of complex formation of nuclear-receptor accessory proteins.
Biochem J. 2000; 345 Pt 3(1-2):627-636

Like other nuclear receptors, steroid hormone receptors form large protein hetero-complexes in their inactive, ligand-friendly state. Several heat-shock proteins, immunophilins and others have been identified as members of these highly dynamic complexes. The interaction kinetics and dynamics of hsp90, hsp70, p60 (Hop), FKBP52, FKBP51, p48 (Hip) and p23 have been assessed by a biosensor approach measuring the complex formation in real time. A core chaperone complex has been reconstituted from p60, hsp90 and hsp70. p60 forms a molecular bridge between hsp90 and hsp70 with an affinity in the range of 10(5) M-1. Dynamics of hsp90-p60 complex formation is modulated by ATP through changes in the co-operativity of interaction. At low protein concentrations ATP stabilizes the complex. Binding of p23 to hsp90 did not change the affinity of the hsp90-p60 complex and the stabilizing effect of ATP. Saturation of the p48-hsp70 interaction could not be achieved, suggesting multiple binding sites. A picture of the protein complex, including stoichiometric coefficients, co-operativity of interaction and equilibrium-binding constants, has been formed.
Authors BOKU Wien:
Jungbauer Alois
Find related publications in this database (using NML MeSH Indexing)
Adenosine Triphosphate - chemistry
Biochemistry - methods
Biosensing Techniques - methods
Carrier Proteins - isolation & purification
Electrophoresis, Polyacrylamide Gel - isolation & purification
Estrogen Receptor alpha - isolation & purification
Glutathione Transferase - genetics
HSP70 Heat-Shock Proteins - isolation & purification
HSP90 Heat-Shock Proteins - metabolism
Heat-Shock Proteins - isolation & purification
Immunoblotting - isolation & purification
Immunophilins - isolation & purification
Kinetics - isolation & purification
Molecular Chaperones - isolation & purification
Nuclear Proteins - isolation & purification
Protein Denaturation - isolation & purification
Receptors, Estrogen - metabolism
Recombinant Proteins - genetics
Surface Plasmon Resonance - genetics
Tacrolimus Binding Proteins - genetics
Tumor Suppressor Proteins - genetics

Find related publications in this database (Keywords)
heat-shock proteins
real-time biological interaction assay
surface plasmon resonance

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