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Bublin, M; Radauer, C; Wilson, IB; Kraft, D; Scheiner, O; Breiteneder, H; Hoffmann-Sommergruber, K.
(2003): Cross-reactive N-glycans of Api g 5, a high molecular weight glycoprotein allergen from celery, are required for immunoglobulin E binding and activation of effector cells from allergic patients.
FASEB J. 2003; 17(12):1697-1699 FullText FullText_BOKU

Allergy diagnosis relying on the determination of specific IgE is frequently complicated by the presence of cross-reacting IgE of unclear clinical relevance. Particularly, the anaphylactogenic activity of IgE directed to cross-reactive carbohydrate moieties of glycoproteins from plants and invertebrates has been a matter of debate. In this study, we present the biochemical and immunological characterization of Api g 5, a glycoprotein allergen from celery with homology to FAD containing oxidases. Carbohydrate analysis of the allergen revealed the presence of glycans carrying fucosyl and xylosyl residues, structures previously shown to bind IgE. Chemical deglycosylation of the protein completely abolished binding of serum IgE from all 14 patients tested. Likewise, basophils from a patient allergic to mugwort pollen and celery were stimulated only by native Api g 5, whereas the deglycosylated allergen did not trigger release of histamine. IgE inhibition immunoblots showed that native Api g 5 other than the deglycosylated protein completely inhibited IgE binding to high molecular weight allergens in protein extracts from birch pollen, mugwort pollen, and celery. A similar inhibition was accomplished using the IgE binding oligosaccharide, MUXF, coupled to bovine serum albumin. All these observations taken together confer convincing evidence that IgE directed to cross-reactive carbohydrates is capable of eliciting allergic reactions in vivo.
Authors BOKU Wien:
Wilson Iain B.H.
Find related publications in this database (using NML MeSH Indexing)
Allergens - chemistry
Apium graveolens - immunology
Basophils - immunology
Cross Reactions - immunology
Epitopes - chemistry
Flavin-Adenine Dinucleotide - analysis
Glycoproteins - chemistry
Glycosylation - chemistry
Histamine Release - chemistry
Humans - chemistry
Hypersensitivity, Immediate - immunology
Immunoglobulin E - immunology
Models, Immunological - immunology
Molecular Weight - immunology
Oxidoreductases - chemistry
Plant Proteins - chemistry
Pollen - immunology
Polysaccharides - chemistry

Find related publications in this database (Keywords)
type I hypersensitivity
food allergy
pollen allergy
berberine bridge enzyme

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