University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Selected Publication:

Hohenblum, H; Borth, N; Mattanovich, D.
(2003): Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry.
J Biotechnol. 2003; 102(3):281-290 FullText FullText_BOKU

This paper describes the establishment of flow cytometric methods for recombinant Pichia pastoris strains, and their application to a lab scale fed batch fermentation. Using a strain which secretes human trypsinogen, the viability and the product which remained associated to the cell were measured with propidium iodide and immunofluorescent staining, respectively. Viability decreases significantly below 70% during the methanol fed batch phase, indicating a stress situation triggered by the fermentation conditions. Cell associated product is accumulated earlier after methanol induction than secreted product. These data demonstrate that flow cytometry is a powerful tool for the analysis and optimization of recombinant protein production processes, and they indicate the need to further improve a widely used fermentation protocol for P. pastoris. (C) 2003 Elsevier Science B.V. All rights reserved.
Authors BOKU Wien:
Borth Nicole
Katinger Hermann
Mattanovich Diethard
Find related publications in this database (using NML MeSH Indexing)
Bioreactors - microbiology
Cell Culture Techniques - methods
Cell Division - methods
Cell Survival - methods
Flow Cytometry - methods
Humans - methods
Methanol - metabolism
Pichia - cytology
Pilot Projects - cytology
Recombinant Proteins - biosynthesis
Trypsinogen - biosynthesis

Find related publications in this database (Keywords)
Pichia pastoris
fed batch
heterologous protein
methanol induction

© BOKU Wien Imprint