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Jisa, E; Jungbauer, A.
(2003): Kinetic analysis of estrogen receptor homo- and heterodimerization in vitro.
J Steroid Biochem Mol Biol. 2003; 84(2-3):141-148 FullText FullText_BOKU

The coexistence of ERalpha and ERbeta suggests that active receptor complexes are present as homo- or heterodimers. In addition each of three forms of active receptors may trigger different cellular responses. A real-time biosensor based on surface plasmon resonance was used as instrument to determine binding kinetics of homo- and heterodimerization of estrogen receptor alpha and beta. Partially purified full-length estrogen receptor alpha was expressed intracellularly as a C-terminal fusion to a hexa-histidine tag using the baculovirus-expression system. Purified estrogen receptor alpha and beta without tags were used as partners in the dimerization process. An association rate constant of 3.6 x 10(3) to 1.5 x 10(4) M-1 s(-1) for the homodimer formation of ERalpha and 5.7 x 10(3) to 1.5 x 10(4) M-1 s(-1) for the heterodimer formation was found assuming a pseudo first-order reaction kinetic. The equilibrium dissociation constant for homodimerization of ERalpha was 2.2 x 10(-8) to 5.4 x 10(-8) and 1.8 x 10(-8) to 2.6 x 10(-8) M for the heterodimer formation. The homo- and heterodimer formation was characterized by a slow association kinetics and kinetic rate constants were within the same range. (C) 2003 Elsevier Science Ltd. All rights reserved.
Authors BOKU Wien:
Jungbauer Alois
Find related publications in this database (using NML MeSH Indexing)
Animals -
Biosensing Techniques -
Cell Line -
Dimerization -
Estradiol - metabolism
Estrogen Receptor alpha - metabolism
Humans - metabolism
Insects - metabolism
Kinetics - metabolism
Ligands - metabolism
Plasmids - metabolism
Protein Binding - metabolism
Protein Structure, Tertiary - metabolism
Receptors, Estrogen - chemistry
Recombinant Proteins - chemistry
Time Factors - chemistry
Transfection - chemistry

Find related publications in this database (Keywords)
human estrogen receptor alpha and beta
17 beta-estradiol

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