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Drs, E., Baumgartner, S., Krska, R., Bremer, M., Kemmers-Voncken, A., Smits, N., Haasnoot, W., Banks, J., Reece, P., Danks, C., Tomkies, V., Immer, U., Schmitt, K..
(2004): Detection of hidden hazelnut proteins in food by IgY-based indirect competitive enzyme-immunoassay
Analytica Chimica Acta, 520, 223-228 FullText FullText_BOKU

Abstract:
The development of an indirect competitive enzyme-immunoassay for the detection of hidden hazelnut protein in complex food matrices is described. A sensitive and selective polyclonal antibody was raised by immunisation of laying hens with protein extracts from roasted hazelnuts. In contrast to traditional antibody generation in mammals, the antibody was not isolated from the blood of immunised mammals but from the egg yolk of immunised chickens. A standard calibration curve was optimised using immunoaffinity purified antibody extract and a coating antigen concentration of 10 mug ml(-1). One percent skim milk powder was chosen for blocking. The assay has a minimum detection limit of 10 mug l(-1), with an IC50 of 618 mug l(-1) when a 50 mM phosphate buffer at pH 7.5 and 10 mM sodium chloride is used as assay buffer. The cross reactivity testing shows a high specificity for hazelnut proteins and various foods and food additives were found to be non reactive except beans, sunflower seed or poppy seed. (C) 2004 Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Baumgartner Sabine
Krska Rudolf

Find related publications in this database (Keywords)
ELISA
hidden allergens
hazelnut
Corylus avellana
egg yolk antibodies
IgY


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