University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Strasser, R; Stadlmann, J; Svoboda, B; Altmann, F; Glössl, J; Mach, L.
(2005):
Molecular basis of N-acetylglucosaminyltransferase I deficiency in Arabidopsis thaliana plants lacking complex N-glycans.
Biochem J. 2005; 387(Pt 2):385-391
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- Abstract:
- GnTI (N-acetylglucosaminyltransferase I) is a Golgi-resident enzyme essential for the processing of high-mannose to hybrid and complex N-glycans. The Arabidopsis thaliana cgl mutant lacks GnTI activity and as a consequence accumulates oligomannosidic structures. Molecular cloning of cgl GnTI cDNA revealed a point mutation, which causes a critical amino acid substitution (Asp(144) -> Asn), thereby creating an additional N-glycosylation site. Heterologous expression of cgl GnTI in insect cells confirmed its lack of activity and the use of the N-glycosylation site. Remarkably, introduction of the Asp(144) -> Asn mutation into rabbit GnTI, which does not result in the formation of a new N-glycosylation site, led to a protein with strongly reduced, but still detectable enzymic activity. Expression of Asn(144) rabbit GnTI in cgl plants could partially restore complex N-glycan formation. These results indicate that the complete deficiency of GnTI activity in cgl plants is mainly due to the additional N-glycan, which appears to interfere with the proper folding of the enzyme.
- Authors BOKU Wien:
- Altmann Friedrich
- Glößl Josef
- Mach Lukas
- Stadlmann Johannes
- Strasser Richard
- BOKU Gendermonitor:
- Find related publications in this database (using NML MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Arabidopsis - enzymology
- Cell Line - enzymology
- Molecular Sequence Data - enzymology
- Mutation - enzymology
- N-Acetylglucosaminyltransferases - genetics
- Plant Leaves - metabolism
- Plants, Genetically Modified - metabolism
- Polysaccharides - metabolism
- Rabbits - metabolism
- Sequence Alignment - metabolism
- Sequence Homology, Amino Acid - metabolism
- Find related publications in this database (Keywords)
- Arabidopsis thaliana
- baculovirus
- N-acetylglucosaminyltransferase I
- N-glycan
- N-glycosylation
- UDP-GlcNAc
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