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Dürrschmid, MP; Landauer, K; Simic, G; Klug, H; Keijzer, T; Trampler, F; Oudshoorn, A; Gröschl, M; Müller, D; Doblhoff-Dier, O.
(2003): Comparison of fluidized bed and ultrasonic cell-retention systems for high cell density mammalian cell culture.
Biotechnol Prog. 2003; 19(3):1045-1048 FullText FullText_BOKU

Economically viable biopharmaceutical production is to a high degree dependent on high product yields and stable fermentation systems that are easy to handle. In the current study we have compared two different fermentation systems for the production of recombinant protein from CHO cells. Both systems are fully scaleable and can be used for industrial high cell density bioprocesses. As a model cell line we have used a recombinant CHO cell line producing the enzyme arylsulfatase B (ASB). CHO cells were cultivated as adherent cell culture attached on Cytoline macroporous microcarrier (Amersham Biosciences, Sweden) using a Cytopilot Mini fluidized bed bioreactor (FBR, Vogelbusch-Amersham Biosciences, Austria) and as suspension culture using a stirred tank bioreactor equipped with a BioSep ultrasonic resonator based cell separation device (Applikon, The Netherlands). Both systems are equally well-suited for stable, long-term high cell density perfusion cell culture and provide industrial scalability and high yields. For products such as the recombinant ASB, high perfusion rates and therefore short product bioreactor residence times may be of additional benefit.
Authors BOKU Wien:
Doblhoff-Dier Otto
Find related publications in this database (using NML MeSH Indexing)
Animals -
Bioreactors -
CHO Cells -
Cell Adhesion - physiology
Cell Culture Techniques - instrumentation
Cell Division - physiology
Cricetinae - physiology
Cricetulus - physiology
Equipment Failure Analysis - physiology
N-Acetylgalactosamine-4-Sulfatase - biosynthesis
Pilot Projects - biosynthesis
Recombinant Proteins - biosynthesis
Rheology - instrumentation
Ultrasonics - instrumentation

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