University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Chang, MW; Grillari, J; Mayrhofer, C; Fortschegger, K; Allmaier, G; Marzban, G; Katinger, H; Voglauer, R.
(2005):
Comparison of early passage, senescent and hTERT immortalized endothelial cells.
Exp Cell Res. 2005; 309(1):121-136
FullText
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- Abstract:
- The need for standardized experimental conditions to gain relevant and reproducible results has increased the demand for well characterized continuously growing cell lines that exhibit the characteristics of their normal counterparts. Immortalization of normal human cells by ectopic expression of the catalytic subunit of human telomerase (hTERT) has shown to result in highly differentiated cell lines. However, the influence of the increased telomerase activity on the protein expression profile was not investigated so far. Therefore, we have immortalized human umbilical vein endothelial cells (HUVECs) by hTERT overexpression and compared them to their normal early passage and senescent counterparts. This study, including a proteomic approach, shows that ectopic hTERT expression leads to a stable growing cell line. Although these cells are highly differentiated, the protein expression profile of the cell line is different to that of normal early passage and senescent cells. (c) 2005 Elsevier Inc. All rights reserved.
- Authors BOKU Wien:
- Grillari Johannes
- Grillari Regina
- Katinger Hermann
- Marzban Gorji
- Find related publications in this database (using NML MeSH Indexing)
- Cell Aging - physiology
- Cell Differentiation - physiology
- Cell Division - physiology
- Cell Line -
- Cell Line, Transformed - physiology
- DNA-Binding Proteins - genetics
- Electrophoresis, Gel, Two-Dimensional -
- Endothelial Cells - cytology
- Gene Expression Regulation -
- Humans -
- Polymerase Chain Reaction -
- Proteomics -
- Telomerase - genetics
- Transfection -
- Find related publications in this database (Keywords)
- endothelial cells
- telomerase
- immortalization
- senescence
- DIGE
- 2-dimensional gel electrophoresis
- real-time PCR
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