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Schabussova, I; Amer, H; van Die, I; Kosma, P; Maizels, RM.
(2007): O-methylated glycans from Toxocara are specific targets for antibody binding in human and animal infections.
Int J Parasitol. 2007; 37(1):97-109 FullText FullText_BOKU

Abstract:
The parasitic helminth Toxocara canis is a widely distributed nematode of mammals. Larval parasites, which infect a wide range of hosts including mice and humans, export glycosylated macromolecules bearing novel methylated oligosaccharide structures, similar to the mammalian blood group antigen H but bearing one or two O-methylated substitutions on the terminal fucose and subterminal galactose residues. We have studied the reactivity of synthetic forms of these glycans to parasite-specific antibodies and mammalian immune system lectins. Murine antibodies, generated to T canis infection, predominantly recognise the mono-O-methylated form with the beta-configuration of the GalNAc residue (MoM beta), and antibodies are entirely IgM isotype. The mAb Tcn-2 reproduces this pattern, and shows little reactivity to either the alpha isomer (MoM alpha) or the di-O-methylated form (DiM). Antibodies generated to helminth infections other than T canis were unreactive with the glycans, except antibodies to other members of the Toxocara genus. Hence, the carbohydrate structures represent immunogenic, genus-specific antigens. Antibodies from human toxocariasis patients are reactive with the same sugars, although preferentially towards DiM. Sera from unrelated helminth infections do not react, confirming the status of these structures as Toxocara-specific glycans. The human dendritic cell lectin, DC-SIGN, was found to bind both Toxocara excretory/secretory products and mammalian blood group antigen H3. However, DC-SIGN did not bind the synthetic glycans, indicating additional non-methylated carbohydrates may also play a role in the interaction between T canis and its host. (c) 2006 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Authors BOKU Wien:
Amer Hassan
Kosma Paul
Find related publications in this database (using NML MeSH Indexing)
Animals -
Antibodies, Helminth - immunology
Antibodies, Monoclonal - immunology
Antibody Specificity - immunology
Cell Adhesion Molecules - immunology
Cross Reactions - immunology
Enzyme-Linked Immunosorbent Assay - methods
Epitopes - immunology
Female - immunology
Helminthiasis - immunology
Hot Temperature - immunology
Humans - immunology
Immunoglobulin M - immunology
Lectins - immunology
Lectins, C-Type - immunology
Methylation - immunology
Mice - immunology
Mice, Inbred BALB C - immunology
Oligosaccharides - immunology
Polysaccharides - immunology
Receptors, Cell Surface - immunology
Toxocara canis - immunology
Toxocariasis - immunology

Find related publications in this database (Keywords)
antibodies
carbohydrate
glycan
helminth
lectin
nematode
oligosaccharide
O-methylation


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