University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Marzban, G; Herndl, A; Maghuly, F; Katinger, H; Laimer, M; .
(2008):
Mapping of fruit allergens by 2D electrophoresis and immunodetection.
Expert Rev Proteomics. 2008; 5(1):61-75
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- Abstract:
- Proteomic analyses of fruits are confronted with a series of specific obstacles: a general low protein content in plant tissues, allergen extraction from highly complex matrices and protein determination in the presence of interfering compounds. Different methods are currently being introduced to achieve higher protein yields and a simultaneous removal of interfering substances, such as polyphenols and polysaccharides. However, no universal protocol suitable for protein purification from any given plant species is available. Protein profiling by 2DE-western blotting offers a powerful tool for the detection and characterization of known and novel plant allergens. Moreover, the detection of IgE-reactive proteins from fruits is improved by combining western blot and alternative visualization techniques. The recent developments in bioinformatics and databases facilitate the interpretation of profiling studies with regard to novel potential fruit allergens.
- Authors BOKU Wien:
- Katinger Hermann
- Laimer Margit
- Maghuly Fatemeh
- Marzban Gorji
- Find related publications in this database (using NML MeSH Indexing)
- Antibodies, Monoclonal/immunology;Antibody Specificity;Antigens, Plant/analysis*;Antigens, Plant/chemistry;Antigens, Plant/immunology;Antigens, Plant/isolation & purification;Blotting, Western/methods*;Cross Reactions;Databases, Protein;Electrophoresis, Gel, Two-Dimensional/methods*;Epitopes/chemistry;Food Hypersensitivity/diagnosis;Food Hypersensitivity/etiology;Fruit/immunology*;Humans;Immunoglobulin E/analysis;Immunoglobulin E/immunology;Iodine Radioisotopes/analysis;Plant Proteins/analysis*;Plant Proteins/chemistry;Plant Proteins/immunology;Plant Proteins/isolation & purification;Pollen/immunology;Serologic Tests/methods;
- Find related publications in this database (Keywords)
- allergen identification
- allergen proteomics
- data mining
- fruit allergen
- fruit protein
- lipid transfer protein
- LTP
- Mal d1
- Mal d3
- profilin
- protein quantification
- Pru ar 1
- Pru ar 3
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