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Selected Publication:

Marzban, G; Herndl, A; Maghuly, F; Katinger, H; Laimer, M; .
(2008): Mapping of fruit allergens by 2D electrophoresis and immunodetection.
Expert Rev Proteomics. 2008; 5(1):61-75 FullText FullText_BOKU

Proteomic analyses of fruits are confronted with a series of specific obstacles: a general low protein content in plant tissues, allergen extraction from highly complex matrices and protein determination in the presence of interfering compounds. Different methods are currently being introduced to achieve higher protein yields and a simultaneous removal of interfering substances, such as polyphenols and polysaccharides. However, no universal protocol suitable for protein purification from any given plant species is available. Protein profiling by 2DE-western blotting offers a powerful tool for the detection and characterization of known and novel plant allergens. Moreover, the detection of IgE-reactive proteins from fruits is improved by combining western blot and alternative visualization techniques. The recent developments in bioinformatics and databases facilitate the interpretation of profiling studies with regard to novel potential fruit allergens.
Authors BOKU Wien:
Katinger Hermann
Laimer Margit
Maghuly Fatemeh
Marzban Gorji
Find related publications in this database (using NML MeSH Indexing)
Antibodies, Monoclonal/immunology;Antibody Specificity;Antigens, Plant/analysis*;Antigens, Plant/chemistry;Antigens, Plant/immunology;Antigens, Plant/isolation & purification;Blotting, Western/methods*;Cross Reactions;Databases, Protein;Electrophoresis, Gel, Two-Dimensional/methods*;Epitopes/chemistry;Food Hypersensitivity/diagnosis;Food Hypersensitivity/etiology;Fruit/immunology*;Humans;Immunoglobulin E/analysis;Immunoglobulin E/immunology;Iodine Radioisotopes/analysis;Plant Proteins/analysis*;Plant Proteins/chemistry;Plant Proteins/immunology;Plant Proteins/isolation & purification;Pollen/immunology;Serologic Tests/methods;

Find related publications in this database (Keywords)
allergen identification
allergen proteomics
data mining
fruit allergen
fruit protein
lipid transfer protein
Mal d1
Mal d3
protein quantification
Pru ar 1
Pru ar 3

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