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Selected Publication:

Gach, JS; Quendler, H; Ferko, B; Katinger, H; Kunert, R.
(2008): Expression, purification, and in vivo administration of a promising anti-idiotypic HIV-1 vaccine.
Mol Biotechnol. 2008; 39(2):119-125 FullText FullText_BOKU

Abstract:
To date only a few neutralizing antibodies against HIV-1 exist. Since these neutralizing antibodies are only rarely found in sera of HIV-1 infected individuals an active vaccine is required. We recently developed murine anti-idiotypic antibody Ab2/3H6 against monoclonal antibody (mAb) 2F5, which is one of the most prominent neutralizing antibodies. Anti-idiotypic antibody Ab2/3H6 has been partially humanized and expressed as whole immunoglobulin G in Chinese hamster ovary cells in order to minimize the human anti-mouse antibody response. Here we describe the expression, purification, and immunohistochemical characterization of the chimeric Ab2/3H6 Fab fragment, which was finally used beside the whole IgG1 as an antigen for immunization of guinea pigs. The crude sera were screened for specific antibodies against the epitope of mAb 2F5 ELDKWA as well as for reactivity against HIV-1 gp41.
Authors BOKU Wien:
Katinger Hermann
Kunert Renate
Quendler Heribert
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
AIDS Vaccines - biosynthesis
Animals -
Antibodies, Anti-Idiotypic - biosynthesis
Antibody Specificity -
CHO Cells -
Chromatography, Gel -
Cricetinae -
Cricetulus -
Electrophoresis, Polyacrylamide Gel -
Enzyme-Linked Immunosorbent Assay -
Glycosylation -
Guinea Pigs -
HIV Antibodies - biosynthesis
HIV-1 - immunology
Immunoglobulin Fab Fragments - biosynthesis
Immunoglobulin G - biosynthesis
Tunicamycin - pharmacology

Find related publications in this database (Keywords)
anti-idiotypic antibodies
HIV-1
2F5
CHO
vaccine


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