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Khaosaad, T; Krenn, L; Medjakovic, S; Ranner, A; Lössl, A; Nell, M; Jungbauer, A; Vierheilig, H.
(2008): Effect of mycorrhization on the isoflavone content and the phytoestrogen activity of red clover.
J Plant Physiol. 2008; 165(11):1161-1167 FullText FullText_BOKU

Red clover, known for its estrogenic activity due to its isoflavones content (biochanin A, genistein, daidzein and formononetin), was inoculated with the arbuscular mycorrhizal fungus Glomus mosseae. Once the symbiotic fungus was well. established, plants were harvested and we determined the root and shoot dry weight as well as the P-content. In roots and leaves the Levels of biochanin A, genistein, daidzein and formononetin were quantified by reversed-phase HPLC and the estrogenic activity of the leaves was measured by a transactivation assay using a yeast two-plasmid system. Mycorrhization increased the Levels of biochanin A in the root and the shoot and reduced the levels of genistein in the shoot of red clover. The levels of the other isoflavones were not affected. The shoot biomass of mycorrhizal plants more than doubled compared with non-mycorrhizal control plants, and this growth-stimulating effect of arbuscular mycorrhiza did not affect the estrogenic activity of red clover. In a control P treatment, the biomass of red clover was greatly enhanced. However, the estrogenic activity was reduced.
Authors BOKU Wien:
Jungbauer Alois
Lössl Andreas
Medjakovic Svjetlana
Nell Monika
Vierheilig Horst
Find related publications in this database (using NML MeSH Indexing)
Biomass -
Genistein - metabolism
Glomeromycota - physiology
Isoflavones - metabolism
Mycorrhizae - physiology
Phosphates - metabolism
Phytoestrogens - metabolism
Plant Leaves - metabolism
Plant Roots - metabolism
Trifolium - metabolism

Find related publications in this database (Keywords)
arbuscular mycorrhiza
red clover

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