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Gruber, P; Gadermaier, G; Bauer, R; Weiss, R; Wagner, S; Leonard, R; Breiteneder, H; Ebner, C; Ferreira, F; Egger, M.
(2009): Role of the polypeptide backbone and post-translational modifications in cross-reactivity of Art v 1, the major mugwort pollen allergen.
Biol Chem. 2009; 390(5-6):445-451 FullText FullText_BOKU

Artemisia vulgaris (mugwort) is one of the main causes of late summer pollinosis in Europe, with >95% of patients sensitized to the glycoallergen Art v 1. Despite the importance of this allergen, little is known about its cross-reactive behavior. Here we investigated the occurrence of conserved Art v 1 antigenic determinants in sources known to display clinically relevant cross-reactivity with mugwort pollen. For this purpose, monoclonal antibodies specific for a cysteine-stabilized epitope of the Art v 1 defensin domain and for carbohydrates attached to the proline domain were produced by hybridoma and phage display technologies. Using polyclonal Art v 1-specific rabbit sera and antibodies against both the Art v 1 carbohydrate and polypeptide moieties, we could identify cross-reactive structures in pollen from botanically related Asteraceae weeds (Artemisia absinthium, Helianthus annuus and Ambrosia sp.). Homologous allergens were also recognized by IgE from mugwort-sensitized patients and the reactivity could be decreased by serum pre-incubation with natural and recombinant Art v 1. As no cross-reactive structures could be found in foods associated with mugwort pollinosis, we conclude that Art v 1 is poorly involved in mugwort cross-reactivity to food allergens.
Authors BOKU Wien:
Leonard Renaud
Find related publications in this database (using NML MeSH Indexing)
Allergens - chemistry
Amino Acid Sequence -
Animals -
Antibodies, Monoclonal - immunology
Artemisia - immunology
Cross Reactions -
Epitopes - chemistry
Humans -
Hybridomas -
Mice -
Mice, Inbred BALB C -
Molecular Sequence Data -
Peptide Library -
Plant Proteins - chemistry
Pollen - chemistry
Protein Processing, Post-Translational -
Rabbits -

Find related publications in this database (Keywords)
ETH-2 phage library
monoclonal antibody
mugwort pollen allergy
pDAP2/S vector
phage display

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