University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Kolbe, T; Holtz, W.
(1999):
Intracytoplasmic injection (ICSI) of in vivo or in vitro matured oocytes with fresh ejaculated or frozen-thawed epididymal spermatozoa and additional calcium-ionophore activation in the pig.
Theriogenology. 1999; 52(4):671-682
FullText
FullText_BOKU
- Abstract:
- In Experiment I, we performed intracytoplasmic sperm injection (ICSI) of frozen-thawed epididymal and fresh ejaculated in vitro-capacitated spermatozoa into in vivo and in vitro-matured porcine oocytes. Within each group, oocytes were sperm-injected, sham-injected or served as handling controls. After subsequent in vitro-culture for 48 h the number of unchanged, fragmented und cleaved oocytes was recorded. The best result (14% cleaved after ICSI vs 2 and 0% with the sham injection and handling controls; P < 0.01) was achieved with fresh in vitro-capacitated spermatozoa injected into in vivo-matured oocytes. Invitro-matured oocytes displayed high fragmentation rates. In Experiment 2, in vitro matured oocytes were injected with freshly ejaculated in vitro-capacitated spermatozoa, followed by a 5 min-exposure to 0 (control), 50 or 100 mu M calcium-ionophore. Comparable groups were sham injected or served as handling controls. It became apparent that Ca-ionophore treatment after injection of spermatozoa was ineffective at 100 mu M, where at 50 mu M a significant reduction in cleavage rate was observed (6 vs 26% with untreated controls, P < 0.01). Fluorescence staining with Hoechst 33342 revealed that in most cases of sperm-injected oocytes that remained unchanged after 48 h of in vitro-culture, sperm heads had not decondensed. Only few oocytes had continued to the pronucleus stage. In this context no favorable effect of Ca-ionophore was to be observed. (C) 1999 by Elsevier Science Inc.
- Authors BOKU Wien:
- Kolbe Thomas
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Calcimycin - pharmacology
- Cryopreservation - veterinary
- Ejaculation -
- Epididymis -
- Female -
- Germ Cells - cytology
- Male -
- Oocytes - cytology
- Semen Preservation - veterinary
- Sperm Capacitation -
- Sperm Injections, Intracytoplasmic - methods
- Spermatozoa - drug effects
- Swine -
- Find related publications in this database (Keywords)
- intracytoplasmic sperm injection
- ICSI
- pig
- Ca-ionophore
- activation
Altmetric: