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Selected Publication:

Schuster, B; Pum, D; Braha, O; Bayley, H; Sleytr, UB; .
(1998): Self-assembled alpha-hemolysin pores in an S-layer-supported lipid bilayer.
Biochim Biophys Acta. 1998; 1370(2):280-288

Abstract:
The effects of a supporting proteinaceous surface-layer (S-layer) from Bacillus coagulans E38-66 on a 1,2-diphytanoyl-sn-glycero-3- phosphatidylcholine (DPhPC) bilayer were investigated. Comparative voltage clamp studies on plain and S-layer supported DPhPC bilayers revealed no significant difference in the capacitance. The conductance of the composite membrane decreased slightly upon recrystallization of the S-layer. Thus, the attached S-layer lattice did not interpenetrate or rupture the DPhPC bilayer. The self-assembly of a pore-forming protein into the S-layer supported lipid bilayer was examined, Staphylococcal alpha-hemolysin formed lytic pores when added to the lipid-exposed side. The assembly was slow compared to unsupported membranes, perhaps due to an altered fluidity of the Lipid bilayer. No assembly could be detected upon adding alpha-hemolysin monomers to the S-layer-faced side of the composite membrane. Therefore. the intrinsic molecular sieving properties of the S-layer lattice do not allow passage of alpha-hemolysin monomers through the S-layer pores to the lipid bilayer. In comparison to plain lipid bilayers, the S-layer supported lipid membrane had a decreased tendency to rupture in the presence of alpha-hemolysin. (C) 1998 Elsevier Science B.V.
Authors BOKU Wien:
Pum Dietmar
Schuster Bernhard
Sleytr Uwe B.
Find related publications in this database (using NML MeSH Indexing)
Bacillus/chemistry;Bacillus/ultrastructure;Bacterial Proteins/chemistry*;Bacterial Proteins/metabolism;Bacterial Toxins/chemistry*;Crystallization;Electric Conductivity;Hemolysin Proteins/chemistry*;Lipid Bilayers/chemistry*;Lipid Bilayers/metabolism;Membrane Glycoproteins/chemistry*;Membrane Glycoproteins/metabolism;Membranes, Artificial;Microscopy, Electron;Phosphatidylcholines/chemistry;Staphylococcus/chemistry;Surface Properties;

Find related publications in this database (Keywords)
crystalline bacterial surface-layer (S-layer)
bilayer lipid membrane technique
electron microscopy
planar biomimetic membrane
voltage clamp


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