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Selected Publication:

Tschiggerl, H; Breitwieser, A; de Roo, G; Verwoerd, T; Schäffer, C; Sleytr, UB.
(2008): Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus.
J Biotechnol. 2008; 133(3):403-411 FullText FullText_BOKU

A fusion protein based on the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and the enzyme laminarinase (LamA) from Pyrococcus furiosus was designed and overexpressed in Escherichia coli. Due to the construction principle, the S-layer fusion protein fully retained the self-assembly capability of the S-layer moiety, while the catalytic domain of LamA remained exposed at the outer surface of the formed protein lattice. The enzyme activity of the S-layer fusion protein monolayer obtained upon recrystallization on silicon wafers, glass slides and different types of polymer membranes was determined colorimetrically and related to the activity of sole LamA that has been immobilized with conventional techniques. LamA aligned within the S-layer fusion protein lattice in a periodic and orientated fashion catalyzed twice the glucose release from the laminarin polysaccharide substrate in comparison to the randomly immobilized enzyme. In combination with the good shelf-life and the high resistance towards temperature and diverse chemicals, these novel composites are regarded a promising approach for site-directed enzyme immobilization. (C) 2007 Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Schäffer Christina
Sleytr Uwe B.
Find related publications in this database (using NML MeSH Indexing)
Archaeal Proteins - isolation & purification
Biotechnology - methods
Catalysis - drug effects
Cellulase - isolation & purification
Cross-Linking Reagents - pharmacology
Crystallization -
Enzyme Stability - drug effects
Enzymes, Immobilized - metabolism
Membrane Glycoproteins - metabolism
Membranes, Artificial -
Pyrococcus furiosus - drug effects
Recombinant Fusion Proteins - ultrastructure
Temperature -

Find related publications in this database (Keywords)
enzyme immobilization
extremophilic enzyme

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