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Selected Publication:

Scheicher, SR; Kainz, B; Köstler, S; Suppan, M; Bizzarri, A; Pum, D; Sleytr, UB; Ribitsch, V.
(2009): Optical oxygen sensors based on Pt(II) porphyrin dye immobilized on S-layer protein matrices.
Biosens Bioelectron. 2009; 25(4):797-802 FullText FullText_BOKU

Abstract:
This paper describes the development of planar and fiber optic oxygen sensors utilizing surface layer (S-layer) proteins as immobilization matrix for oxygen sensitive dyes. S-layer proteins have the intrinsic capability to reassemble into two-dimensional arrays in suspension and at interfaces. Due to their crystalline character the distribution of functional groups, such as carboxylic groups, is repeated with the periodicity of the lattice and thus allows the reproducible and geometrically distinct binding of functional molecules. For the development of oxygen sensors an oxygen sensitive Pt(II) porphyrin dye was covalently bound to the S-layer matrix. Measurement of the oxygen concentration was performed by phase modulation fluorimetry. Setups comprising low cost optoelectronic components like LEDs and silicon photodiodes were constructed. For both sensor setups (planar and fiber optic) variations in the oxygen concentrations resulted in distinct and reproducible changes in luminescence lifetime and intensity. The luminescence quenching efficiency of these sensors was found to be 1.5-1.9 (expressed as the ratio of signal under nitrogen and air) which compares well to other sensor systems using luminophores embedded in polymer matrices. These results demonstrated the application potential of S-layers as immobilization matrices in the development of (bio-)sensors. (C) 2009 Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Pum Dietmar
Sleytr Uwe B.
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Adsorption -
Binding Sites -
Biosensing Techniques - instrumentation
Equipment Design -
Equipment Failure Analysis -
Fluorometry - instrumentation
Membrane Glycoproteins - chemistry
Optical Devices -
Oxygen - analysis
Porphyrins - chemistry
Protein Binding -

Find related publications in this database (Keywords)
S-layer protein
Fiber optic sensor
Oxygen sensor
Phase modulation fluorimetry
Immobilization matrix


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