University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Apellaniz, B; García-Sáez, AJ; Huarte, N; Kunert, R; Vorauer-Uhl, K; Katinger, H; Schwille, P; Nieva, JL.
(2010):
Confocal microscopy of giant vesicles supports the absence of HIV-1 neutralizing 2F5 antibody reactivity to plasma membrane phospholipids.
FEBS Lett. 2010; 584(8):1591-1596
FullText
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- Abstract:
- The broadly neutralizing anti-HIV-12F5 monoclonal antibody recognizes a gp41 epitope proximal to the viral membrane. Potential phospholipid autoreactivity at cell surfaces has raised concerns about the use of this antibody for development of vaccines or immunotherapy. In this study, confocal microscopy of giant unilamellar vesicles (GUVs) was used to assess 2F5 reactivity with phospholipids assembled into bilayers with surface charge and curvature stress approximating those of the eukaryotic plasma membranes. Antibody partitioning into lipid bilayers required the specific recognition of membrane-inserted epitope, indicating that 2F5 was unable to directly react with GUV phospholipids, even under fluid phase segregation conditions. Our results thus support the feasibility of raising 2F5-like neutralizing responses through vaccination, and the medical safety of mAb infusions. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- Authors BOKU Wien:
- Katinger Hermann
- Kunert Renate
- Vorauer-Uhl Karola
- Find related publications in this database (using NML MeSH Indexing)
- Amino Acid Sequence -
- Antibodies, Monoclonal - immunology
- Antibodies, Neutralizing - immunology
- Antibody Specificity -
- Cell Membrane - metabolism
- Epitopes - immunology
- Flow Cytometry -
- HIV-1 - immunology
- Lipid Bilayers - metabolism
- Membrane Microdomains - metabolism
- Microscopy, Confocal -
- Molecular Sequence Data -
- Peptides - chemistry
- Phospholipids - metabolism
- Physicochemical Processes -
- Unilamellar Liposomes - metabolism
- Find related publications in this database (Keywords)
- Passive immunotherapy
- HIV-1 neutralization
- 2F5
- gp41
- Membrane-proximal external region
- Peptide vaccine
- Broadly neutralizing antibody
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