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Selected Publication:

Apellaniz, B; García-Sáez, AJ; Huarte, N; Kunert, R; Vorauer-Uhl, K; Katinger, H; Schwille, P; Nieva, JL.
(2010): Confocal microscopy of giant vesicles supports the absence of HIV-1 neutralizing 2F5 antibody reactivity to plasma membrane phospholipids.
FEBS Lett. 2010; 584(8):1591-1596 FullText FullText_BOKU

The broadly neutralizing anti-HIV-12F5 monoclonal antibody recognizes a gp41 epitope proximal to the viral membrane. Potential phospholipid autoreactivity at cell surfaces has raised concerns about the use of this antibody for development of vaccines or immunotherapy. In this study, confocal microscopy of giant unilamellar vesicles (GUVs) was used to assess 2F5 reactivity with phospholipids assembled into bilayers with surface charge and curvature stress approximating those of the eukaryotic plasma membranes. Antibody partitioning into lipid bilayers required the specific recognition of membrane-inserted epitope, indicating that 2F5 was unable to directly react with GUV phospholipids, even under fluid phase segregation conditions. Our results thus support the feasibility of raising 2F5-like neutralizing responses through vaccination, and the medical safety of mAb infusions. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Katinger Hermann
Kunert Renate
Vorauer-Uhl Karola
Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Antibodies, Monoclonal - immunology
Antibodies, Neutralizing - immunology
Antibody Specificity -
Cell Membrane - metabolism
Epitopes - immunology
Flow Cytometry -
HIV-1 - immunology
Lipid Bilayers - metabolism
Membrane Microdomains - metabolism
Microscopy, Confocal -
Molecular Sequence Data -
Peptides - chemistry
Phospholipids - metabolism
Physicochemical Processes -
Unilamellar Liposomes - metabolism

Find related publications in this database (Keywords)
Passive immunotherapy
HIV-1 neutralization
Membrane-proximal external region
Peptide vaccine
Broadly neutralizing antibody

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