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Selected Publication:

Lim, K; Ho, JX; Keeling, K; Gilliland, GL; Ji, X; Rüker, F; Carter, DC.
(1994): Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV.
Protein Sci. 1994; 3(12):2233-2244 FullText FullText_BOKU

Abstract:
The 3-dimensional crystal structure of glutathione S-transferase (GST) of Schistosoma japonicum (Sj) fused with a conserved neutralizing epitope on gp41 (glycoprotein, 41 kDa) of human immunodeficiency virus type 1 (HIV-1) (Muster T et al., 1993, J Virol 67:6642-6647) was determined at 2.5 Angstrom resolution. The structure of the 3-3 isozyme rat GST of the mu gene class (Ji X, Zhang P, Armstrong RN, Gilliland GL, 1992, Biochemistry 31:10169-10184) was used as a molecular replacement model. The structure consists of a 4-stranded beta-sheet and 3 alpha-helices in domain 1 and 5 alpha-helices in domain 2. The space group of the Sj GST crystal is P4(3)2(1)2, with unit cell dimensions of a = b = 94.7 Angstrom, and c = 58.1 Angstrom. The crystal has 1 GST monomer per asymmetric unit, and 2 monomers that form an active dimer are related by crystallographic 2-fold symmetry. In the binding site, the ordered structure of reduced glutathione is observed. The gp41 peptide (Glu-Leu-Asp-Lys-Trp-Ala) fused to the C-terminus of Sj GST forms a loop stabilized by symmetry-related GSTs. The Sj GST structure is compared with previously determined GST structures of mammalian gene classes mu, alpha, and pi. Conserved amino acid residues among the 4 GSTs that are important for hydrophobic and hydrophilic interactions for dimer association and glutathione binding are discussed.
Authors BOKU Wien:
Rüker Florian
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Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Animals -
Antibodies, Monoclonal - immunology
Binding Sites -
Chemistry, Physical -
Crystallography, X-Ray -
Epitopes - chemistry
Glutathione -
Glutathione Transferase - metabolism
HIV Envelope Protein gp41 - chemistry
HIV-1 - genetics
Helminth Proteins - chemistry
Models, Molecular -
Molecular Sequence Data -
Physicochemical Phenomena -
Protein Conformation -
Protein Structure, Tertiary -
Rats -
Recombinant Fusion Proteins - chemistry
Schistosoma japonicum - enzymology
Sequence Alignment -
Sequence Homology, Amino Acid -

Find related publications in this database (Keywords)
FUSION PROTEIN
GLUTATHIONE S-TRANSFERASE
HUMAN IMMUNODEFICIENCY VIRUS TYPE 1
SCHISTOSOMA JAPONICUM
3-DIMENSIONAL STRUCTURE


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