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Aziz, MG; Michlmayr, H; Kulbe, KD; Del Hierro, AM; .
(2011): Biotransformation of pineapple juice sugars into dietetic derivatives by using a cell free oxidoreductase from Zymomonas mobilis together with commercial invertase.
Enzyme Microb Technol. 2011; 48(1):85-91 FullText FullText_BOKU

Abstract:
An easy procedure for cell free biotransformation of pineapple juice sugars into dietetic derivatives was accomplished using a commercial invertase and an oxidoreductase from Zymomonas mobilis. First, pineapple juice sucrose was quantitatively converted into glucose and fructose by invertase, thus increasing the concentration of each monosaccharide in the original juice to almost twice. In a second step, glucose-fructose oxidoreductase (GFOR) transformed glucose into gluconolactone, and fructose into the low calorie sweetener sorbitol. The advantage of using GFOR is simultaneous reduction of fructose and oxidation of glucose, allowing the continuous regeneration of the essential coenzyme NADP(H), that is tightly bound to the enzyme. The yield of GFOR catalyzed sugar conversion depends on initial pH and control of pH during the reaction. At optimal conditions (pH control at 6.2) a maximum of 80% (w/v) sugar conversion was obtained. Without pH control, GFOR is inactivated rapidly due to gluconic acid formation. Therefore, conversion yields are relatively low at the natural pH of pineapple juice. The application of this process might be more advantageous on juices of other tropical fruits (papaya, jackfruit, mango) due to their naturally given higher pH. (C) 2010 Elsevier Inc. All rights reserved.
Authors BOKU Wien:
Del Hierro Belleza Andrès Miguel
Kulbe Klaus Dieter
Michlmayr Herbert
Find related publications in this database (using NML MeSH Indexing)
Ananas/metabolism*;Beverages/microbiology*;Biotransformation;Carbohydrates/chemistry;Dietary Carbohydrates/metabolism*;Food Technology/methods*;Fructose/metabolism;Glucose/metabolism;Oxidation-Reduction;Oxidoreductases/metabolism*;Sorbitol/metabolism;Zymomonas/enzymology*;beta-Fructofuranosidase/metabolism*;

Find related publications in this database (Keywords)
Pineapple juice
Glucose-fructose oxidoreductase
Calorie reduction
Sucrose
Gluconolactone
Invertase


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