University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Karola, VU; Dominik, J; Gabriele, L; Rainer, H; Andreas, W; Dietmar, K.
(2011):
IMPACT OF ALCOHOLIC SOLVENTS ON THE RECOVERY OF PHOSPHOLIPIDS IN HPLC ANALYSIS
J LIQ CHROMATOGR RELAT TECHNO. 2011; 34(3): 217-230.
FullText
FullText_BOKU
- Abstract:
- The lipid quantification of liposomal preparations is primarily performed by HPLC analysis; therefore, reversed phase and normal phase HPLC techniques have been successfully developed. While the optimization of separation performances and the establishment of appropriate detection systems have been studied in detail, the matrix effect of the aqueous environment of liposomes has been less recognized, although this might significantly affect the final results of individual lipids used in liposomal formulations. A series of solubilization studies were performed to prove the effect of increasing water amount in certain lipid standards. Calibration curves of selected lipids were established in pure methanol, isopropanol, and additionally in both solvents containing 10% water for the lipid quantification of liposomal samples. Furthermore, the physical properties of the selected solvents were correlated with the calibration curves to identify their contribution to the magnitude of the peak areas. By employing this experimental design we could demonstrate that the recovery of individual lipids by RP-HPLC is heavily dependent on the solvent itself as well as the final water content of the sample. It could be identified that for DOTAP, the density of the solvent is the main effective parameter. Therefore, to obtain reliable results of liposomal suspensions either the related calibration stock solutions need to be adjusted properly to the solute matrix or alternatively quantification can be performed with the established model.
- Authors BOKU Wien:
- Hahn Rainer
- Jeschek Dominik
- Lhota Gabriele
- Vorauer-Uhl Karola
- Find related publications in this database (Keywords)
- cholesterol
- DOTAP
- lipid-quantification
- liposomes
- phospholipids
- reversed phase HPLC
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