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Dellago, H; Löscher, M; Ajuh, P; Ryder, U; Kaisermayer, C; Grillari-Voglauer, R; Fortschegger, K; Gross, S; Gstraunthaler, A; Borth, N; Eisenhaber, F; Lamond, AI; Grillari, J.
(2011): Exo70, a subunit of the exocyst complex, interacts with SNEV(hPrp19/hPso4) and is involved in pre-mRNA splicing.
Biochem J. 2011; 438(1):81-91 FullText FullText_BOKU

The Cdc5L (cell division cycle 5-like) complex is a spliceosomal subcomplex that also plays a role in DNA repair. The complex contains the splicing factor hPrp19, also known as SNEV or hPso4, which is involved in cellular life-span regulation and proteasomal breakdown. In a recent large-scale proteomics analysis for proteins associated with this complex, proteins involved in transcription, cell-cycle regulation, DNA repair, the ubiquitin-proteasome system, chromatin remodelling, cellular aging, the cytoskeleton and trafficking, including four members of the exocyst complex, were identified. In the present paper we report that Exo70 interacts directly with SNEV(hPrp19/hPso4) and shuttles to the nucleus, where it associates with the spliceosome. We mapped the interaction site to the N-terminal 100 amino acids of Exo70, which interfere with pre-mRNA splicing in vitro. Furthermore, Exo70 influences the splicing of a model substrate as well as of its own pre-mRNA in vivo. In addition, we found that Exo70 is alternatively spliced in a cell-type- and cell-age-dependent way. These results suggest a novel and unexpected role of Exo70 in nuclear mRNA splicing, where it might signal membrane events to the splicing apparatus.
Authors BOKU Wien:
Borth Nicole
Dellago Hanna
Grillari Johannes
Grillari Regina
Find related publications in this database (using NML MeSH Indexing)
Alternative Splicing -
Blotting, Western -
Cell Nucleus - genetics
DNA Repair Enzymes - genetics
Fluorescent Antibody Technique -
HeLa Cells -
Humans -
Molecular Sequence Data -
Nuclear Proteins - genetics
Protein Binding -
RNA Precursors - genetics
RNA, Messenger - genetics
Reverse Transcriptase Polymerase Chain Reaction -
Spliceosomes - genetics
Two-Hybrid System Techniques -
Vesicular Transport Proteins - genetics

Find related publications in this database (Keywords)
alternative splicing
protein interaction

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