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Berski, S; van Bergeijk, J; Schwarzer, D; Stark, Y; Kasper, C; Scheper, T; Grothe, C; Gerardy-Schahn, R; Kirschning, A; Dräger, G.
(2008): Synthesis and biological evaluation of a polysialic acid-based hydrogel as enzymatically degradable scaffold material for tissue engineering.
Biomacromolecules. 2008; 9(9):2353-2359 FullText FullText_BOKU

Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC 12 cells. Here, additional coating with collagen 1, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
Authors BOKU Wien:
Almeria Ciarra
Kasper Cornelia
Find related publications in this database (using NML MeSH Indexing)
Animals -
Cell Adhesion - drug effects
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured -
Collagen - chemistry
Collagen Type I - chemistry
Drug Combinations -
Electrophoresis, Polyacrylamide Gel -
Hydrogels - chemical synthesis
Hydrolysis -
Laminin - chemistry
Molecular Conformation -
Neuraminidase - metabolism
PC12 Cells -
Polylysine - chemistry
Proteoglycans - chemistry
Rats -
Sialic Acids - metabolism
Tissue Engineering - methods

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