University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Berski, S; van Bergeijk, J; Schwarzer, D; Stark, Y; Kasper, C; Scheper, T; Grothe, C; Gerardy-Schahn, R; Kirschning, A; Dräger, G.
(2008):
Synthesis and biological evaluation of a polysialic acid-based hydrogel as enzymatically degradable scaffold material for tissue engineering.
Biomacromolecules. 2008; 9(9):2353-2359
FullText
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- Abstract:
- Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC 12 cells. Here, additional coating with collagen 1, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
- Authors BOKU Wien:
- Almeria Ciarra
- Kasper Cornelia
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Cell Adhesion - drug effects
- Cell Proliferation - drug effects
- Cell Survival - drug effects
- Cells, Cultured -
- Collagen - chemistry
- Collagen Type I - chemistry
- Drug Combinations -
- Electrophoresis, Polyacrylamide Gel -
- Hydrogels - chemical synthesis
- Hydrolysis -
- Laminin - chemistry
- Molecular Conformation -
- Neuraminidase - metabolism
- PC12 Cells -
- Polylysine - chemistry
- Proteoglycans - chemistry
- Rats -
- Sialic Acids - metabolism
- Tissue Engineering - methods
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