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Selected Publication:

Lebuhn, M; Effenberger, M; Gronauer, A; Wilderer, PA; Wuertz, S.
(2003): Using quantitative real-time PCR to determine the hygienic status of cattle manure.
Water Sci Technol. 2003; 48(4):97-103

We developed a suitable system of DNA extraction and real-time quantitative polymerase chain reaction (qPCR) for the specific and sensitive quantification of pathogens and other relevant (indicator) organisms in recalcitrant material such as cattle manure. PCR inhibition by coextraction of humic compounds was minimized in this system, resulting in detection sensitivity of one target DNA copy per reaction well. Data from qPCR analysis for Escherichia coli agreed with cultivation based results, but orders of magnitude more fecal enterococci, Enterobacteriaceae and Campylobacter jejuni, were determined by qPCR than by cultivation. These bacteria may have been in a potentially hazardous active but non-cultivable state. The qPCR system is much less time consuming than conventional cultivation, highly specific, can detect non-cultivable organisms, provides high measurement throughput, and is cost attractive. It should be considered as an alternative in various application areas for (prescribed routine) cultivation based assays, e.g. for biosafety and hygiene monitoring.
Authors BOKU Wien:
Gronauer Andreas
Find related publications in this database (using NML MeSH Indexing)
Animals -
Bacteria, Anaerobic -
Bioreactors -
Campylobacter jejuni - genetics
Cattle -
DNA, Bacterial - analysis
Enterobacteriaceae - genetics
Environmental Monitoring - methods
Escherichia coli - genetics
Hygiene -
Manure - microbiology
Polymerase Chain Reaction -
Safety -
Sensitivity and Specificity -

Find related publications in this database (Keywords)
anaerobic digestion
DNA extraction
PCR inhibitors
quantitative real-time PCR

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